Projects per year
Abstract
Meiotic chromosomes are organized into linear looped chromatin arrays by a protein axis localized along the loop-bases. Programmed remodelling of the axis occurs during prophase I of meiosis. Structured illumination microscopy (SIM) has revealed dynamic changes in the chromosome axis in Arabidopsis thaliana and Brassica oleracea. We show that the axis associated protein ASY1 is depleted during zygotene concomitant with synaptonemal complex (SC) formation. Study of an Atpch2 mutant demonstrates this requires the conserved AAA+ ATPase, PCH2, which localizes to the sites of axis remodelling. Loss of PCH2 leads to a failure to deplete ASY1 from the axes and compromizes SC polymerisation. Immunolocalization of recombination proteins in Atpch2 indicates that recombination initiation and CO designation during early prophase I occur normally. Evidence suggests that CO interference is initially functional in the mutant but there is a defect in CO maturation following designation. This leads to a reduction in COs and a failure to form COs between some homologous chromosome pairs leading to univalent chromosomes at metaphase I. Genetic analysis reveals that CO distribution is also affected in some chromosome regions. Together these data indicate that the axis remodelling defect in Atpch2 disrupts normal patterned formation of COs.
Original language | English |
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Article number | e1005372 |
Journal | PLoS Genetics |
Volume | 11 |
Issue number | 7 |
DOIs | |
Publication status | Published - 16 Jul 2015 |
Keywords
- Arabidopsis thaliana
- Recombinant proteins
- Prophase
- Chromosomes
- crossover interference
- DNA recombination
- Saccharomyces cerevisiae
- Genetic interference
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Dive into the research topics of 'Arabidopsis PCH2 mediates meiotic chromosome remodeling and maturation of crossovers'. Together they form a unique fingerprint.Projects
- 2 Finished
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ERA-CAPS 13 Delineating the crossover control networks in plants (DeCOP)
Franklin, C. (Principal Investigator) & Sanchez-Moran, E. (Co-Investigator)
Biotechnology & Biological Sciences Research Council
1/04/14 → 30/09/17
Project: Research Councils
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Coordination of meiotic recombination and prophase I progression in plants: the role of retinoblastoma (RBR)
Franklin, C. (Principal Investigator), Sanchez-Moran, E. (Co-Investigator) & Higgins, J. (Co-Investigator)
Biotechnology & Biological Sciences Research Council
30/06/13 → 31/08/16
Project: Research