Abstract
Dynamic nuclear polarization (DNP) has become an attractive technique to boost the sensitivity of NMR experiments. In the case of ex situ polarizations two-dimensional (2D) spectra are limited by the short lifetime of the polarization after dissolution and sample transfer to a high field NMR magnet. This limitation can be overcome by various approaches. Here we show how the use of 13C-labelled acetyl tags can help to obtain 2D-HMQC spectra for many small molecules, owing to a nuclear Overhauser enhancement between 13C spins originating from the long-lived carbonyl carbon, which extends the lifetimes of other 13C spins with shorter longitudinal relaxation times. We also show an alternative approach of using an optimized polarization matrix.
Original language | English |
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Pages (from-to) | 5868-5871 |
Number of pages | 4 |
Journal | Physical Chemistry Chemical Physics |
Volume | 12 |
Issue number | 22 |
Early online date | 10 May 2010 |
DOIs | |
Publication status | Published - 14 Jun 2010 |
Keywords
- NR4A nuclear receptors
- Orphan nuclear receptor
- Parkinson's disease
- Nurr1
- NMR