Application of dual Nr4a1-GFP Nr4a3-Tocky reporter mice to study T cell receptor signaling by flow cytometry

Emma Jennings; David Lecky; Masahiro Ono; David Bending

doi:10.1016/j.xpro.2020.100284

Application of dual Nr4a1-GFP Nr4a3-Tocky reporter mice to study T cell receptor signaling by flow cytometry

Emma Jennings, David Lecky, Masahiro Ono, David Bending

Immunology and Immunotherapy

Research output: Contribution to journal › Article › peer-review

244 Downloads (Pure)

Abstract

This protocol uses Nr4a1-GFP Nr4a3-Tocky mice to study T cell receptor (TCR) signaling using flow cytometry. It identifies the optimal mouse transgenic status and fluorochromes compatible with the dual reporter. This protocol has applications in TCR signaling, and we outline how to obtain high-quality datasets. It is not compatible with cellular fixation, and cells should be analyzed immediately after staining.

For complete details on the use and execution of this protocol, please refer to Jennings et al., 2020.

Original language	English
Article number	100284
Journal	STAR Protocols
Volume	2
Issue number	1
Early online date	21 Jan 2021
DOIs	https://doi.org/10.1016/j.xpro.2020.100284
Publication status	Published - 19 Mar 2021

Access to Document

10.1016/j.xpro.2020.100284Licence: Creative Commons: Attribution (CC BY)

JenningsEK2021ApplicationFinal published version, 1.82 MBLicence: Creative Commons: Attribution (CC BY)

1 Finished
1 Active

Negative feedback control of T cells in tolerance and cancer - from pathways to biomarkers
Bending, D.
Medical Research Council
1/02/21 → 31/01/26
Project: Research Councils
How do T Cell Receptor signalling dynamics regulate interleukin-10-producing T-cell differentiation?
Bending, D.
THE WELLCOME TRUST
5/11/18 → 5/05/21
Project: Research

Cite this

@article{9550809f513a4e6889a08f0f557b7b8f,

title = "Application of dual Nr4a1-GFP Nr4a3-Tocky reporter mice to study T cell receptor signaling by flow cytometry",

abstract = "This protocol uses Nr4a1-GFP Nr4a3-Tocky mice to study T cell receptor (TCR) signaling using flow cytometry. It identifies the optimal mouse transgenic status and fluorochromes compatible with the dual reporter. This protocol has applications in TCR signaling, and we outline how to obtain high-quality datasets. It is not compatible with cellular fixation, and cells should be analyzed immediately after staining. For complete details on the use and execution of this protocol, please refer to Jennings et al., 2020. ",

author = "Emma Jennings and David Lecky and Masahiro Ono and David Bending",

year = "2021",

month = mar,

day = "19",

doi = "10.1016/j.xpro.2020.100284",

language = "English",

volume = "2",

journal = "STAR Protocols",

issn = "2666-1667",

publisher = "Elsevier",

number = "1",

}

TY - JOUR

T1 - Application of dual Nr4a1-GFP Nr4a3-Tocky reporter mice to study T cell receptor signaling by flow cytometry

AU - Jennings, Emma

AU - Lecky, David

AU - Ono, Masahiro

AU - Bending, David

PY - 2021/3/19

Y1 - 2021/3/19

N2 - This protocol uses Nr4a1-GFP Nr4a3-Tocky mice to study T cell receptor (TCR) signaling using flow cytometry. It identifies the optimal mouse transgenic status and fluorochromes compatible with the dual reporter. This protocol has applications in TCR signaling, and we outline how to obtain high-quality datasets. It is not compatible with cellular fixation, and cells should be analyzed immediately after staining. For complete details on the use and execution of this protocol, please refer to Jennings et al., 2020.

AB - This protocol uses Nr4a1-GFP Nr4a3-Tocky mice to study T cell receptor (TCR) signaling using flow cytometry. It identifies the optimal mouse transgenic status and fluorochromes compatible with the dual reporter. This protocol has applications in TCR signaling, and we outline how to obtain high-quality datasets. It is not compatible with cellular fixation, and cells should be analyzed immediately after staining. For complete details on the use and execution of this protocol, please refer to Jennings et al., 2020.

U2 - 10.1016/j.xpro.2020.100284

DO - 10.1016/j.xpro.2020.100284

M3 - Article

C2 - 33532733

SN - 2666-1667

VL - 2

JO - STAR Protocols

JF - STAR Protocols

IS - 1

M1 - 100284

ER -

Application of dual Nr4a1-GFP Nr4a3-Tocky reporter mice to study T cell receptor signaling by flow cytometry

Abstract

Access to Document

Fingerprint

Projects

Negative feedback control of T cells in tolerance and cancer - from pathways to biomarkers

How do T Cell Receptor signalling dynamics regulate interleukin-10-producing T-cell differentiation?

Cite this