Antimicrobial peptide coatings for hydroxyapatite: electrostatic and covalent attachment of antimicrobial peptides to surfaces

Leigh Townsend, Richard Williams, Olachi Anuforom, Matthew Berwick, Fenella Halstead, Erik Hughes, Artemis Stamboulis, Beryl Oppenheim, Julie E. Gough, Liam Grover, Robert Scott, Mark Webber, Anna Peacock, Antonio Belli, Ann Logan, Felicity De Cogan

Research output: Contribution to journalArticlepeer-review

25 Citations (Scopus)
174 Downloads (Pure)

Abstract

The interface between implanted devices and their host tissue is complex and is often optimized for maximal integration and cell adhesion. However, this also gives a surface suitable for bacterial colonization. We have developed a novel method of modifying the surface at the material–tissue interface with an antimicrobial peptide (AMP) coating to allow cell attachment while inhibiting bacterial colonization. The technology reported here is a dual AMP coating. The dual coating consists of AMPs covalently bonded to the hydroxyapatite surface, followed by deposition of electrostatically bound AMPs. The dual approach gives an efficacious coating which is stable for over 12 months and can prevent colonization of the surface by both Gram-positive and Gram-negative bacteria.
Original languageEnglish
Article number20160657
Number of pages12
JournalJournal of The Royal Society Interface
Volume14
Issue number126
Early online date1 Jan 2017
DOIs
Publication statusPublished - 31 Jan 2017

Bibliographical note

© 2017 The Author(s).

Keywords

  • Animals
  • Antimicrobial Cationic Peptides/chemistry
  • Cell Line
  • Coated Materials, Biocompatible/chemistry
  • Durapatite/chemistry
  • Gram-Negative Bacteria/growth & development
  • Gram-Positive Bacteria/growth & development
  • Materials Testing
  • Mice
  • Osteoblasts/cytology
  • Static Electricity

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