Antimicrobial efficacy of different irrigant solutions using a novel biofilm model: an in vitro confocal laser scanning microscopy experiment

Satnam Virdee, Firas Saddam Oglah Albaaj, Josette Camilleri, Melissa Grant, Damien Walmsley, Paul Cooper, Phillip Tomson

Research output: Contribution to journalArticlepeer-review

176 Downloads (Pure)


Aim: To determine the ability of different irrigation solutions to biomechanically remove Enterococcus faecalis biofilm from a novel artificial root canal model during chemomechanical preparation.

Methods: High resolution micro-computer-tomography scans of a mandibular molar's mesial root were used to produce 50 identical 3D-printed resin root canal models. These were cultured with E.faecalis over seven days to generate biofilm and subjected to chemomechanical preparation using: saline; 17% ethylenediaminetetraacetic acid (EDTA) or 2% sodium hypochlorite (NaOCl) alongside positive/negative controls (n = 10). Canals were prepared to 40/.06 taper, with 1 mL irrigation between instruments, followed by 5 mL penultimate rinse, 30 s ultrasonic activation and 5 mL final rinse. Residual biofilm volume (pixels) was determined following immunofluorescent staining and confocal-laser-scanning-microscopy imaging. Statistical comparisons were made using Kruskal-Wallis with post-hoc Dunn's tests (α ⟨0.05).

Results: In all canal thirds, the greatest biofilm removal was observed with NaOCl, followed by EDTA and saline. The latter had significantly higher E.faecalis counts than NaOCl and EDTA (P ⟨0.01). However, no statistical differences were found between EDTA and NaOCl or saline and positive controls (P ⟩0.05).

Conclusions: Within limitations of this model, 17% EDTA was found to be as effective as 2% NaOCl at eradicating E.faecalis biofilm following chemomechanical preparation. Further investigations with multi-species biofilms are encouraged.
Original languageEnglish
JournalThe European journal of prosthodontics and restorative dentistry
Early online date13 Jul 2022
Publication statusE-pub ahead of print - 13 Jul 2022


  • Biofilm
  • CLSM
  • EDTA
  • Enterococcus Faecalis
  • Irrigants
  • Sodium Hypochlorite


Dive into the research topics of 'Antimicrobial efficacy of different irrigant solutions using a novel biofilm model: an in vitro confocal laser scanning microscopy experiment'. Together they form a unique fingerprint.

Cite this