Angiogenic Activity of Dentin Matrix Components

R Zhang; Paul Cooper; Gay Smith; JE Noer; Anthony Smith

doi:10.1016/j.joen.2010.08.042

Angiogenic Activity of Dentin Matrix Components

R Zhang, Paul Cooper, Gay Smith, JE Noer, Anthony Smith

Dentistry

Research output: Contribution to journal › Article

70 Citations (Scopus)

Abstract

Angiogenesis is key to both the development and regeneration of the dentin-pulp complex. Objective: We hypothesized that proangiogenic signaling molecules sequestered in dentin matrix can be solubilised to induce angiogenic events. Methods: Matrix components were extracted from powdered sound human dentin with EDTA and their dose-dependent (0.0001-5 mg/mL) effects examined in endothelial cells in an in vitro angiogenic tube formation assay, proliferation assay, and transcriptional regulation of the VEGF and VEGF-R2 genes. Results: Lower concentrations of dentin matrix components were found to show proangiogenic activity, whereas higher concentrations suppressed angiogenic activity. Conclusion: This study highlights that the release of dentin matrix components after dental injury can contribute to the angiogenic events that support pulp regeneration. (J Endod 2011;37:26-30)

Original language	English
Pages (from-to)	26-30
Number of pages	5
Journal	Journal of Endodontics
Volume	37
Issue number	1
DOIs	https://doi.org/10.1016/j.joen.2010.08.042
Publication status	Published - 1 Jan 2011

Keywords

dentin
revascularization
regeneration
stem cells
Angiogenesis
endodontics
growth factors

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10.1016/j.joen.2010.08.042

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abstract = "Angiogenesis is key to both the development and regeneration of the dentin-pulp complex. Objective: We hypothesized that proangiogenic signaling molecules sequestered in dentin matrix can be solubilised to induce angiogenic events. Methods: Matrix components were extracted from powdered sound human dentin with EDTA and their dose-dependent (0.0001-5 mg/mL) effects examined in endothelial cells in an in vitro angiogenic tube formation assay, proliferation assay, and transcriptional regulation of the VEGF and VEGF-R2 genes. Results: Lower concentrations of dentin matrix components were found to show proangiogenic activity, whereas higher concentrations suppressed angiogenic activity. Conclusion: This study highlights that the release of dentin matrix components after dental injury can contribute to the angiogenic events that support pulp regeneration. (J Endod 2011;37:26-30)",

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author = "R Zhang and Paul Cooper and Gay Smith and JE Noer and Anthony Smith",

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doi = "10.1016/j.joen.2010.08.042",

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T1 - Angiogenic Activity of Dentin Matrix Components

AU - Zhang, R

AU - Cooper, Paul

AU - Smith, Gay

AU - Noer, JE

AU - Smith, Anthony

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AB - Angiogenesis is key to both the development and regeneration of the dentin-pulp complex. Objective: We hypothesized that proangiogenic signaling molecules sequestered in dentin matrix can be solubilised to induce angiogenic events. Methods: Matrix components were extracted from powdered sound human dentin with EDTA and their dose-dependent (0.0001-5 mg/mL) effects examined in endothelial cells in an in vitro angiogenic tube formation assay, proliferation assay, and transcriptional regulation of the VEGF and VEGF-R2 genes. Results: Lower concentrations of dentin matrix components were found to show proangiogenic activity, whereas higher concentrations suppressed angiogenic activity. Conclusion: This study highlights that the release of dentin matrix components after dental injury can contribute to the angiogenic events that support pulp regeneration. (J Endod 2011;37:26-30)

KW - dentin

KW - revascularization

KW - regeneration

KW - stem cells

KW - Angiogenesis

KW - endodontics

KW - growth factors

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DO - 10.1016/j.joen.2010.08.042

M3 - Article

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VL - 37

SP - 26

EP - 30

JO - Journal of Endodontics

JF - Journal of Endodontics

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ER -

Angiogenic Activity of Dentin Matrix Components

Abstract

Keywords

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