Analysis of tamoxifen, N-desmethyltamoxifen and 4-hydroxytamoxifen levels in cytosol and KCl-nuclear extracts of breast tumours from tamoxifen treated patients by gas chromatography-mass spectrometry (GC-MS) using selected ion monitoring (SIM)

C. Murphy; T. Fotsis; P. Pantzar; H. Adlercreutz; F. Martin

doi:10.1016/0022-4731(87)90387-6

Analysis of tamoxifen, N-desmethyltamoxifen and 4-hydroxytamoxifen levels in cytosol and KCl-nuclear extracts of breast tumours from tamoxifen treated patients by gas chromatography-mass spectrometry (GC-MS) using selected ion monitoring (SIM)

C. Murphy, T. Fotsis, P. Pantzar, H. Adlercreutz, F. Martin

Biosciences

Research output: Contribution to journal › Article › peer-review

34 Citations (Scopus)

Abstract

Tamoxifen, 4-hydroxytamoxifen and desmethyltamoxifen levels were measured in cytosolic and 0.5 M KCl extracted nuclear fractions from a small series of breast tumours from tamoxifen treated patients by gas chromatography-mass spectrometry (GC-MS) using selected ion monitoring (SIM). Tamoxifen and desmethyltamoxifen were the most abundant metabolites. There was a small increment in the relative abundance of 4-hydroxytamoxifen in the nuclear extract over cytosol relative to both tamoxifen and desmethyltamoxifen. Further, there was a selective retention of tamoxifen relative to desmethyltamoxifen in the nuclear extract relative to the cytosol. It is concluded that all three compounds could potentially contribute to estrogen receptor mediated antiestrogenic effects in this target tissue.

Original language	English
Pages (from-to)	609-618
Number of pages	10
Journal	Journal of Steroid Biochemistry
Volume	28
Issue number	6
DOIs	https://doi.org/10.1016/0022-4731(87)90387-6
Publication status	Published - Dec 1987

Bibliographical note

M1 - 6

Murphy, C Fotsis, T Pantzar, P Adlercreutz, H Martin, F Research Support, Non-U.S. Gov't England Journal of steroid biochemistry J Steroid Biochem. 1987 Dec;28(6):609-18.

Keywords

Breast Neoplasms/*analysis Cell Nucleus/analysis Cytosol/analysis Female Gas Chromatography-Mass Spectrometry Humans Potassium Chloride/pharmacology Tamoxifen/*analogs & derivatives/*analysis

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Murphy, C., Fotsis, T., Pantzar, P., Adlercreutz, H., & Martin, F. (1987). Analysis of tamoxifen, N-desmethyltamoxifen and 4-hydroxytamoxifen levels in cytosol and KCl-nuclear extracts of breast tumours from tamoxifen treated patients by gas chromatography-mass spectrometry (GC-MS) using selected ion monitoring (SIM). Journal of Steroid Biochemistry, 28(6), 609-618. https://doi.org/10.1016/0022-4731(87)90387-6

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title = "Analysis of tamoxifen, N-desmethyltamoxifen and 4-hydroxytamoxifen levels in cytosol and KCl-nuclear extracts of breast tumours from tamoxifen treated patients by gas chromatography-mass spectrometry (GC-MS) using selected ion monitoring (SIM)",

abstract = "Tamoxifen, 4-hydroxytamoxifen and desmethyltamoxifen levels were measured in cytosolic and 0.5 M KCl extracted nuclear fractions from a small series of breast tumours from tamoxifen treated patients by gas chromatography-mass spectrometry (GC-MS) using selected ion monitoring (SIM). Tamoxifen and desmethyltamoxifen were the most abundant metabolites. There was a small increment in the relative abundance of 4-hydroxytamoxifen in the nuclear extract over cytosol relative to both tamoxifen and desmethyltamoxifen. Further, there was a selective retention of tamoxifen relative to desmethyltamoxifen in the nuclear extract relative to the cytosol. It is concluded that all three compounds could potentially contribute to estrogen receptor mediated antiestrogenic effects in this target tissue.",

keywords = "Breast Neoplasms/*analysis Cell Nucleus/analysis Cytosol/analysis Female Gas Chromatography-Mass Spectrometry Humans Potassium Chloride/pharmacology Tamoxifen/*analogs & derivatives/*analysis",

author = "C. Murphy and T. Fotsis and P. Pantzar and H. Adlercreutz and F. Martin",

note = "M1 - 6 Murphy, C Fotsis, T Pantzar, P Adlercreutz, H Martin, F Research Support, Non-U.S. Gov't England Journal of steroid biochemistry J Steroid Biochem. 1987 Dec;28(6):609-18.",

year = "1987",

month = dec,

doi = "10.1016/0022-4731(87)90387-6",

language = "English",

volume = "28",

pages = "609--618",

journal = "Journal of Steroid Biochemistry",

issn = "0022-4731",

publisher = "Pergamon Press Ltd.",

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Murphy, C, Fotsis, T, Pantzar, P, Adlercreutz, H & Martin, F 1987, 'Analysis of tamoxifen, N-desmethyltamoxifen and 4-hydroxytamoxifen levels in cytosol and KCl-nuclear extracts of breast tumours from tamoxifen treated patients by gas chromatography-mass spectrometry (GC-MS) using selected ion monitoring (SIM)', Journal of Steroid Biochemistry, vol. 28, no. 6, pp. 609-618. https://doi.org/10.1016/0022-4731(87)90387-6

Analysis of tamoxifen, N-desmethyltamoxifen and 4-hydroxytamoxifen levels in cytosol and KCl-nuclear extracts of breast tumours from tamoxifen treated patients by gas chromatography-mass spectrometry (GC-MS) using selected ion monitoring (SIM). / Murphy, C.; Fotsis, T.; Pantzar, P. et al.
In: Journal of Steroid Biochemistry, Vol. 28, No. 6, 12.1987, p. 609-618.

Research output: Contribution to journal › Article › peer-review

TY - JOUR

T1 - Analysis of tamoxifen, N-desmethyltamoxifen and 4-hydroxytamoxifen levels in cytosol and KCl-nuclear extracts of breast tumours from tamoxifen treated patients by gas chromatography-mass spectrometry (GC-MS) using selected ion monitoring (SIM)

AU - Murphy, C.

AU - Fotsis, T.

AU - Pantzar, P.

AU - Adlercreutz, H.

AU - Martin, F.

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N2 - Tamoxifen, 4-hydroxytamoxifen and desmethyltamoxifen levels were measured in cytosolic and 0.5 M KCl extracted nuclear fractions from a small series of breast tumours from tamoxifen treated patients by gas chromatography-mass spectrometry (GC-MS) using selected ion monitoring (SIM). Tamoxifen and desmethyltamoxifen were the most abundant metabolites. There was a small increment in the relative abundance of 4-hydroxytamoxifen in the nuclear extract over cytosol relative to both tamoxifen and desmethyltamoxifen. Further, there was a selective retention of tamoxifen relative to desmethyltamoxifen in the nuclear extract relative to the cytosol. It is concluded that all three compounds could potentially contribute to estrogen receptor mediated antiestrogenic effects in this target tissue.

AB - Tamoxifen, 4-hydroxytamoxifen and desmethyltamoxifen levels were measured in cytosolic and 0.5 M KCl extracted nuclear fractions from a small series of breast tumours from tamoxifen treated patients by gas chromatography-mass spectrometry (GC-MS) using selected ion monitoring (SIM). Tamoxifen and desmethyltamoxifen were the most abundant metabolites. There was a small increment in the relative abundance of 4-hydroxytamoxifen in the nuclear extract over cytosol relative to both tamoxifen and desmethyltamoxifen. Further, there was a selective retention of tamoxifen relative to desmethyltamoxifen in the nuclear extract relative to the cytosol. It is concluded that all three compounds could potentially contribute to estrogen receptor mediated antiestrogenic effects in this target tissue.

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Murphy C, Fotsis T, Pantzar P, Adlercreutz H, Martin F. Analysis of tamoxifen, N-desmethyltamoxifen and 4-hydroxytamoxifen levels in cytosol and KCl-nuclear extracts of breast tumours from tamoxifen treated patients by gas chromatography-mass spectrometry (GC-MS) using selected ion monitoring (SIM). Journal of Steroid Biochemistry. 1987 Dec;28(6):609-618. doi: 10.1016/0022-4731(87)90387-6