Analysis of expressed sequence tags (ESTs) from the green alga Ulva linza (Chlorophyta)

Michele Stanley, Ruth Perry, James Callow

Research output: Contribution to journalArticle

26 Citations (Scopus)


There is a general lack of genomic information available for chlorophyte seaweed genera such as Ulva, and in particular there is no information concerning the genes that contribute to adhesion and cell wall biosynthesis for this organism. Partial sequencing of cDNA libraries to generate expressed sequence tags (ESTs) is an effective means of gene discovery and characterization of expression patterns. In this study, a cDNA library was created from sporulating tissue of Ulva linza L. Initially, 650 ESTs were randomly selected from a cDNA library and sequenced from their 5' ends to obtain an indication of the level of redundancy of the library (21%). The library was normalized to enrich for rarer sequences, and a further 1920 ESTs were sequenced. These sequences were subjected to contig assembly that resulted in a unigene set of approximately 1104 ESTs. Forty-eight percent of these sequences exhibited significant similarity to sequences in the databases. Phylogenetic comparisons are made between selected sequences with similarity in the databases to proteins involved in aspects of extracellular matrix/cell wall assembly and adhesion.
Original languageEnglish
Pages (from-to)1219-1226
Number of pages8
JournalJournal of Phycology
Issue number6
Publication statusPublished - 1 Dec 2005


  • Chlorophyta
  • cell wall
  • adhesion
  • Ulva linza
  • genomics
  • EST (expressed sequence tags)


Dive into the research topics of 'Analysis of expressed sequence tags (ESTs) from the green alga Ulva linza (Chlorophyta)'. Together they form a unique fingerprint.

Cite this