TY - JOUR
T1 - Analysis of CD34+ subsets in stem cell harvests can more reliably predict rapidity and durability of engraftment than total CD34+ cell dose but steady state levels do not correlate with bone marrow reserve
AU - Pratt, Guy
AU - Rawstron, AC
AU - English, AE
AU - Johnson, Roderick
AU - Jack, AS
AU - Morgan, GJ
AU - Smith, GM
PY - 2001/9/1
Y1 - 2001/9/1
N2 - In peripheral blood stem cell transplantation (PBSCT), the number of CD34+ cells transplanted has been shown to correlate well with both rapidity and durability of engraftment. However, it is clear that engraftment does not necessarily correlate with total CD34+ cell numbers in some patients. Consequently, there is increasing interest in evaluating the role of CD34+ subsets in haemopoietic recovery as a more accurate marker of harvest quality. We analysed the numbers of CD34+ cell subsets, namely Thy-1+, L-Selectin+ and CD38-, and correlated this with engraftment in 86 patients undergoing PBSCT. Adequate engraftment was defined as being a platelet count greater than 50 x 10(9)/l and a neutrophil count greater than 1.0 x 10(9)/l. CD34+L-Selectin+ provided the best prediction of engraftment rapidity, although the improvement over total CD34+ cell dose was minor. Only the dose of CD34+Thy-1+ cells transplanted correlated with durable engraftment. The probability of adequate 3-month engraftment increased with the dose of CD34+ cells transplanted, but 10% of patients receiving > 5 x 10(6)/kg still showed poor engraftment at 3 months. However, all patients receiving > 2.5 x 10(5)/kg CD34+Thy-1+ showed adequate engraftment at this time point. We also demonstrated that CD34+Thy-1+ progenitors were restricted to the bone marrow under normal conditions and, during stem cell mobilization, their kinetics generally paralleled total CD34+ numbers.
AB - In peripheral blood stem cell transplantation (PBSCT), the number of CD34+ cells transplanted has been shown to correlate well with both rapidity and durability of engraftment. However, it is clear that engraftment does not necessarily correlate with total CD34+ cell numbers in some patients. Consequently, there is increasing interest in evaluating the role of CD34+ subsets in haemopoietic recovery as a more accurate marker of harvest quality. We analysed the numbers of CD34+ cell subsets, namely Thy-1+, L-Selectin+ and CD38-, and correlated this with engraftment in 86 patients undergoing PBSCT. Adequate engraftment was defined as being a platelet count greater than 50 x 10(9)/l and a neutrophil count greater than 1.0 x 10(9)/l. CD34+L-Selectin+ provided the best prediction of engraftment rapidity, although the improvement over total CD34+ cell dose was minor. Only the dose of CD34+Thy-1+ cells transplanted correlated with durable engraftment. The probability of adequate 3-month engraftment increased with the dose of CD34+ cells transplanted, but 10% of patients receiving > 5 x 10(6)/kg still showed poor engraftment at 3 months. However, all patients receiving > 2.5 x 10(5)/kg CD34+Thy-1+ showed adequate engraftment at this time point. We also demonstrated that CD34+Thy-1+ progenitors were restricted to the bone marrow under normal conditions and, during stem cell mobilization, their kinetics generally paralleled total CD34+ numbers.
UR - http://www.scopus.com/inward/record.url?scp=0034786919&partnerID=8YFLogxK
U2 - 10.1046/j.1365-2141.2001.03027.x
DO - 10.1046/j.1365-2141.2001.03027.x
M3 - Article
C2 - 11564089
SN - 1365-2141
SN - 1365-2141
SN - 1365-2141
SN - 1365-2141
SN - 1365-2141
SN - 1365-2141
SN - 1365-2141
SN - 1365-2141
SN - 1365-2141
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SN - 1365-2141
SN - 1365-2141
SN - 1365-2141
SN - 1365-2141
SN - 1365-2141
SN - 1365-2141
SN - 1365-2141
SN - 1365-2141
SN - 1365-2141
SN - 1365-2141
SN - 1365-2141
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SN - 1365-2141
SN - 1365-2141
SN - 1365-2141
SN - 1365-2141
SN - 1365-2141
SN - 1365-2141
SN - 1365-2141
SN - 1365-2141
SN - 1365-2141
SN - 1365-2141
SN - 1365-2141
SN - 1365-2141
SN - 1365-2141
SN - 1365-2141
SN - 1365-2141
SN - 1365-2141
SN - 1365-2141
SN - 1365-2141
SN - 1365-2141
SN - 1365-2141
SN - 1365-2141
SN - 1365-2141
SN - 1365-2141
SN - 1365-2141
SN - 1365-2141
SN - 1365-2141
SN - 1365-2141
SN - 1365-2141
SN - 1365-2141
SN - 1365-2141
SN - 1365-2141
SN - 1365-2141
SN - 1365-2141
SN - 1365-2141
SN - 1365-2141
SN - 1365-2141
SN - 1365-2141
SN - 1365-2141
SN - 1365-2141
SN - 1365-2141
VL - 114
SP - 937
EP - 943
JO - British Journal of Haematology
JF - British Journal of Haematology
ER -