TY - JOUR
T1 - An unusual structure at one end of potato potyvirus particles
AU - Torrance, Lesley
AU - Gabrenaite-Verhovskaya, Rasa
AU - Cowan, Graham
AU - Mäkinen, Kristiina
AU - Taliansky, Michael E
AU - Andreyev, Igor
PY - 2006/3/17
Y1 - 2006/3/17
N2 - The particles of potato virus Y (PVY) and potato virus A (PVA) potyviruses are helically constructed filaments that are thought to contain a single type of coat protein subunit. Examination of negatively stained virions by electron microscopy reveals flexuous rod-shaped particles with no obvious terminal structures. It is known that some helically constructed rod-shaped virus particles incorporate additional minor protein components, which form stable complexes that mediate particle disassembly, movement or transmission by vectors. Some of this information has been obtained using imaging techniques such as atomic force microscopy. The particles of PVY and PVA were examined by atomic force microscopy and immunogold labelling electron microscopy. Our results show that some of the potyvirus particles contain a protruding tip at one end of the virus particles, which is presumably associated with the 5'-end of viral RNA. The tip contains the virus-encoded proteins genome-linked protein and helper-component proteinase. The composition and possible roles of the terminal tip structures in virus biology are discussed.
AB - The particles of potato virus Y (PVY) and potato virus A (PVA) potyviruses are helically constructed filaments that are thought to contain a single type of coat protein subunit. Examination of negatively stained virions by electron microscopy reveals flexuous rod-shaped particles with no obvious terminal structures. It is known that some helically constructed rod-shaped virus particles incorporate additional minor protein components, which form stable complexes that mediate particle disassembly, movement or transmission by vectors. Some of this information has been obtained using imaging techniques such as atomic force microscopy. The particles of PVY and PVA were examined by atomic force microscopy and immunogold labelling electron microscopy. Our results show that some of the potyvirus particles contain a protruding tip at one end of the virus particles, which is presumably associated with the 5'-end of viral RNA. The tip contains the virus-encoded proteins genome-linked protein and helper-component proteinase. The composition and possible roles of the terminal tip structures in virus biology are discussed.
KW - Immunohistochemistry
KW - Microscopy, Atomic Force
KW - Potyvirus
KW - Protein Conformation
KW - Viral Proteins
UR - https://www.scopus.com/pages/publications/33644776775
U2 - 10.1016/j.jmb.2005.12.021
DO - 10.1016/j.jmb.2005.12.021
M3 - Article
C2 - 16414068
SN - 0022-2836
VL - 357
SP - 1
EP - 8
JO - Journal of Molecular Biology
JF - Journal of Molecular Biology
IS - 1
ER -