An integrated approach for genome annotation of the eukaryotic thermophile Chaetomium thermophilum

Thomas Bock, Wei-Hua Chen, Alessandro Ori, Nayab Malik, Noella Silva-Martin, Jaime Huerta-Cepas, Sean T Powell, Panagiotis L Kastritis, Georgy Smyshlyaev, Ivana Vonkova, Joanna Kirkpatrick, Tobias Doerks, Leo Nesme, Jochen Baßler, Martin Kos, Ed Hurt, Teresa Carlomagno, Anne-Claude Gavin, Orsolya Barabas, Christoph W MüllerVera van Noort, Martin Beck, Peer Bork

Research output: Contribution to journalArticlepeer-review

Abstract

The thermophilic fungus Chaetomium thermophilum holds great promise for structural biology. To increase the efficiency of its biochemical and structural characterization and to explore its thermophilic properties beyond those of individual proteins, we obtained transcriptomics and proteomics data, and integrated them with computational annotation methods and a multitude of biochemical experiments conducted by the structural biology community. We considerably improved the genome annotation of Chaetomium thermophilum and characterized the transcripts and expression of thousands of genes. We furthermore show that the composition and structure of the expressed proteome of Chaetomium thermophilum is similar to its mesophilic relatives. Data were deposited in a publicly available repository and provide a rich source to the structural biology community.

Original languageEnglish
Pages (from-to)13525-33
Number of pages9
JournalNucleic Acids Research
Volume42
Issue number22
DOIs
Publication statusPublished - 16 Dec 2014

Bibliographical note

© The Author(s) 2014. Published by Oxford University Press on behalf of Nucleic Acids Research.

Keywords

  • Chaetomium/genetics
  • Fungal Proteins/genetics
  • Genes, Fungal
  • Genome, Fungal
  • Introns
  • Molecular Sequence Annotation
  • Proteome/metabolism
  • Pseudogenes
  • Transcriptome

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