An in vitro model for the development of mature bone containing an osteocyte network

Alexandra Iordachescu, Harsh Amin, Sara Rankin, Richard Williams, Clarence Yapp, Alistair Bannerman, Alexandra Pacureanu, Owen Addison, Philippa Hulley, Liam Grover

Research output: Contribution to journalArticlepeer-review

8 Citations (Scopus)
162 Downloads (Pure)


Bone is a dynamic tissue that remodels continuously in response to local mechanical and chemical stimuli. This process can also result in maladaptive ectopic bone in response to injury, yet pathological differences at the molecular and structural levels are poorly understood. A number of in vivo models exist but can often be too complex to allow isolation of factors which may stimulate disease progression. A self-structuring model of bone formation is presented using a fibrin gel cast between two calcium phosphate ceramic anchors. Femoral periosteal cells, seeded into these structures, deposit an ordered matrix that closely resembles mature bone in terms of chemistry (collagen:mineral ratio) and structure, which is adapted over a period of one year in culture. Raman spectroscopy and X-ray diffraction confirm that the mineral is hydroxyapatite associated with collagen. Second-harmonic imaging demonstrates that collagen is organized similarly to mature mouse femora. Remarkably, cells differentiated to the osteocyte phase are linked by canaliculi (as demonstrated with nano-computed tomography) and remained viable over the full year of culture. It is demonstrated that novel drugs can prevent ossification in constructs. This model can be employed to study bone formation in an effort to encourage or prevent ossification in a range of pathologies.
Original languageEnglish
Article number1700156
Number of pages12
JournalAdvanced Biosystems
Issue number2
Early online date22 Dec 2017
Publication statusPublished - Feb 2018


Dive into the research topics of 'An in vitro model for the development of mature bone containing an osteocyte network'. Together they form a unique fingerprint.

Cite this