Ambient surface mass spectrometry-ion mobility spectrometry of intact proteins

Rian Griffiths; Klaudia Kocurek; Helen Cooper

doi:10.1016/j.cbpa.2017.11.002

Ambient surface mass spectrometry-ion mobility spectrometry of intact proteins

Rian Griffiths, Klaudia Kocurek, Helen Cooper

Biosciences

Research output: Contribution to journal › Article › peer-review

5 Citations (Scopus)

152 Downloads (Pure)

Abstract

Ambient surface mass spectrometry encompasses a broad range of sampling and ionization techniques. To date, only a small subset of these, based on liquid microjunction extraction, have proven suitable for intact protein analysis from thin tissue sections. Liquid extraction surface analysis shows particular promise for this application. Recently, a range of ion mobility spectrometry approaches have been coupled with ambient mass spectrometry. Improvements in signal-to-noise ratios, decreased chemical noise and separation of molecular classes have been described for the analysis of various biological substrates. Similar benefits have been described for ambient mass spectrometry imaging studies. In this review, we discuss the application of ambient mass spectrometry and ion mobility spectrometry to the analysis of intact proteins, and discuss opportunities and challenges for the field.

Original language	English
Pages (from-to)	67-75
Journal	Current Opinion in Chemical Biology
Volume	42
Early online date	16 Jan 2018
DOIs	https://doi.org/10.1016/j.cbpa.2017.11.002
Publication status	Published - Feb 2018

Access to Document

10.1016/j.cbpa.2017.11.002Licence: Creative Commons: Attribution (CC BY)

Griffiths_et_al_Ambient_surface_mass_spectrometry-ion_mobility_COCBFinal published version, 836 KBLicence: Creative Commons: Attribution (CC BY)

Cite this

@article{0ff0a4e725754809b153e309f5977480,

title = "Ambient surface mass spectrometry-ion mobility spectrometry of intact proteins",

abstract = "Ambient surface mass spectrometry encompasses a broad range of sampling and ionization techniques. To date, only a small subset of these, based on liquid microjunction extraction, have proven suitable for intact protein analysis from thin tissue sections. Liquid extraction surface analysis shows particular promise for this application. Recently, a range of ion mobility spectrometry approaches have been coupled with ambient mass spectrometry. Improvements in signal-to-noise ratios, decreased chemical noise and separation of molecular classes have been described for the analysis of various biological substrates. Similar benefits have been described for ambient mass spectrometry imaging studies. In this review, we discuss the application of ambient mass spectrometry and ion mobility spectrometry to the analysis of intact proteins, and discuss opportunities and challenges for the field.",

author = "Rian Griffiths and Klaudia Kocurek and Helen Cooper",

year = "2018",

month = feb,

doi = "10.1016/j.cbpa.2017.11.002",

language = "English",

volume = "42",

pages = "67--75",

journal = "Current Opinion in Chemical Biology",

issn = "1367-5931",

publisher = "Elsevier",

}

TY - JOUR

T1 - Ambient surface mass spectrometry-ion mobility spectrometry of intact proteins

AU - Griffiths, Rian

AU - Kocurek, Klaudia

AU - Cooper, Helen

PY - 2018/2

Y1 - 2018/2

N2 - Ambient surface mass spectrometry encompasses a broad range of sampling and ionization techniques. To date, only a small subset of these, based on liquid microjunction extraction, have proven suitable for intact protein analysis from thin tissue sections. Liquid extraction surface analysis shows particular promise for this application. Recently, a range of ion mobility spectrometry approaches have been coupled with ambient mass spectrometry. Improvements in signal-to-noise ratios, decreased chemical noise and separation of molecular classes have been described for the analysis of various biological substrates. Similar benefits have been described for ambient mass spectrometry imaging studies. In this review, we discuss the application of ambient mass spectrometry and ion mobility spectrometry to the analysis of intact proteins, and discuss opportunities and challenges for the field.

AB - Ambient surface mass spectrometry encompasses a broad range of sampling and ionization techniques. To date, only a small subset of these, based on liquid microjunction extraction, have proven suitable for intact protein analysis from thin tissue sections. Liquid extraction surface analysis shows particular promise for this application. Recently, a range of ion mobility spectrometry approaches have been coupled with ambient mass spectrometry. Improvements in signal-to-noise ratios, decreased chemical noise and separation of molecular classes have been described for the analysis of various biological substrates. Similar benefits have been described for ambient mass spectrometry imaging studies. In this review, we discuss the application of ambient mass spectrometry and ion mobility spectrometry to the analysis of intact proteins, and discuss opportunities and challenges for the field.

U2 - 10.1016/j.cbpa.2017.11.002

DO - 10.1016/j.cbpa.2017.11.002

M3 - Article

C2 - 29166625

SN - 1367-5931

VL - 42

SP - 67

EP - 75

JO - Current Opinion in Chemical Biology

JF - Current Opinion in Chemical Biology

ER -

Ambient surface mass spectrometry-ion mobility spectrometry of intact proteins

Abstract

Access to Document

Fingerprint

Cite this