AftD functions as an α1→5 arabinofuranosyltransferase involved in the biosynthesis of the mycobacterial cell wall core

Luke J. Alderwick; Helen L. Birch; Karin Krumbach; Michael Bott; Lothar Eggeling; Gurdyal S. Besra

doi:10.1016/j.tcsw.2017.10.001

AftD functions as an α1→5 arabinofuranosyltransferase involved in the biosynthesis of the mycobacterial cell wall core

Luke J. Alderwick, Helen L. Birch, Karin Krumbach, Michael Bott, Lothar Eggeling, Gurdyal S. Besra

Biosciences

Research output: Contribution to journal › Article › peer-review

7 Citations (Scopus)

185 Downloads (Pure)

Abstract

Arabinogalactan (AG) is an essential structural macromolecule present in the cell wall of Mycobacterium tuberculosis, serving to connect peptidoglycan with the outer mycolic acid layer. The D-arabinan segment is a highly branched component of AG and is assembled in a step-wise fashion by a variety of arabinofuranosyltransferases (AraT). We have previously used Corynebacterium glutamicum as a model organism to study these complex processes which are otherwise essential in mycobacteria. In order to further our understanding of the molecular basis of AG assembly, we investigated the role of a fourth AraT, now termed AftD by generating single (ΔaftD) and double deletion (ΔaftB ΔaftD) mutants of C. glutamicum. We demonstrate that AftD functions as an α(1→5) AraT and reveal the point at which it exerts its activity in the AG biosynthetic pathway.

Original language	English
Pages (from-to)	2-14
Journal	The Cell Surface
Volume	1
Early online date	1 Dec 2017
DOIs	https://doi.org/10.1016/j.tcsw.2017.10.001
Publication status	Published - Mar 2018

Keywords

Corynebacterium glutamicum
Mycobacterium tuberculosis
cell wall
Arabinogalacan
Glycosyltransferase

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

Access to Document

10.1016/j.tcsw.2017.10.001Licence: Creative Commons: Attribution (CC BY)

Alderwick_et_al_2018_AftD_functions_Cell_SurfaceFinal published version, 2 MBLicence: Creative Commons: Attribution (CC BY)

http://www.sciencedirect.com/science/article/pii/S2468233017300671Licence: Creative Commons: Attribution (CC BY)

An integrated multi-disciplinary approach to unravel complex and essential cell wall biosynthetic pathways
Besra, D. (Principal Investigator) & Futterer, K. (Co-Investigator)
Wellcome Trust
1/01/08 → 30/06/13
Project: Research

Cite this

@article{518b4677638d425fa5dda5b7ba01a77c,

title = "AftD functions as an α1→5 arabinofuranosyltransferase involved in the biosynthesis of the mycobacterial cell wall core",

abstract = "Arabinogalactan (AG) is an essential structural macromolecule present in the cell wall of Mycobacterium tuberculosis, serving to connect peptidoglycan with the outer mycolic acid layer. The D-arabinan segment is a highly branched component of AG and is assembled in a step-wise fashion by a variety of arabinofuranosyltransferases (AraT). We have previously used Corynebacterium glutamicum as a model organism to study these complex processes which are otherwise essential in mycobacteria. In order to further our understanding of the molecular basis of AG assembly, we investigated the role of a fourth AraT, now termed AftD by generating single (ΔaftD) and double deletion (ΔaftB ΔaftD) mutants of C. glutamicum. We demonstrate that AftD functions as an α(1→5) AraT and reveal the point at which it exerts its activity in the AG biosynthetic pathway.",

keywords = "Corynebacterium glutamicum , Mycobacterium tuberculosis , cell wall , Arabinogalacan , Glycosyltransferase",

author = "Alderwick, {Luke J.} and Birch, {Helen L.} and Karin Krumbach and Michael Bott and Lothar Eggeling and Besra, {Gurdyal S.}",

year = "2018",

month = mar,

doi = "10.1016/j.tcsw.2017.10.001",

language = "English",

volume = "1",

pages = "2--14",

journal = "The Cell Surface",

issn = "2468-2330",

publisher = "Elsevier",

}

TY - JOUR

T1 - AftD functions as an α1→5 arabinofuranosyltransferase involved in the biosynthesis of the mycobacterial cell wall core

AU - Alderwick, Luke J.

AU - Birch, Helen L.

AU - Krumbach, Karin

AU - Bott, Michael

AU - Eggeling, Lothar

AU - Besra, Gurdyal S.

PY - 2018/3

Y1 - 2018/3

N2 - Arabinogalactan (AG) is an essential structural macromolecule present in the cell wall of Mycobacterium tuberculosis, serving to connect peptidoglycan with the outer mycolic acid layer. The D-arabinan segment is a highly branched component of AG and is assembled in a step-wise fashion by a variety of arabinofuranosyltransferases (AraT). We have previously used Corynebacterium glutamicum as a model organism to study these complex processes which are otherwise essential in mycobacteria. In order to further our understanding of the molecular basis of AG assembly, we investigated the role of a fourth AraT, now termed AftD by generating single (ΔaftD) and double deletion (ΔaftB ΔaftD) mutants of C. glutamicum. We demonstrate that AftD functions as an α(1→5) AraT and reveal the point at which it exerts its activity in the AG biosynthetic pathway.

AB - Arabinogalactan (AG) is an essential structural macromolecule present in the cell wall of Mycobacterium tuberculosis, serving to connect peptidoglycan with the outer mycolic acid layer. The D-arabinan segment is a highly branched component of AG and is assembled in a step-wise fashion by a variety of arabinofuranosyltransferases (AraT). We have previously used Corynebacterium glutamicum as a model organism to study these complex processes which are otherwise essential in mycobacteria. In order to further our understanding of the molecular basis of AG assembly, we investigated the role of a fourth AraT, now termed AftD by generating single (ΔaftD) and double deletion (ΔaftB ΔaftD) mutants of C. glutamicum. We demonstrate that AftD functions as an α(1→5) AraT and reveal the point at which it exerts its activity in the AG biosynthetic pathway.

KW - Corynebacterium glutamicum

KW - Mycobacterium tuberculosis

KW - cell wall

KW - Arabinogalacan

KW - Glycosyltransferase

U2 - 10.1016/j.tcsw.2017.10.001

DO - 10.1016/j.tcsw.2017.10.001

M3 - Article

C2 - 29998212

SN - 2468-2330

VL - 1

SP - 2

EP - 14

JO - The Cell Surface

JF - The Cell Surface

ER -

AftD functions as an α1→5 arabinofuranosyltransferase involved in the biosynthesis of the mycobacterial cell wall core

Abstract

Keywords

UN SDGs

Access to Document

Fingerprint

Projects

An integrated multi-disciplinary approach to unravel complex and essential cell wall biosynthetic pathways

Cite this