Adsorption of a styrene maleic acid (SMA) copolymer-stabilized phospholipid nanodisc on a solid-supported planar lipid bilayer

Stephen C.l. Hall, Luke A. Clifton, Cecilia Tognoloni, Kerrie A. Morrison, Timothy J. Knowles, Christian J. Kinane, Tim R. Dafforn, Karen J. Edler, Thomas Arnold

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Abstract

Over recent years, there has been a rapid development of membrane-mimetic systems to encapsulate and stabilize planar segments of phospholipid bilayers in solution. One such system has been the use of amphipathic copolymers to solubilize lipid bilayers into nanodiscs. The attractiveness of this system, in part, stems from the capability of these polymers to solubilize membrane proteins directly from the host cell membrane. The assumption has been that the native lipid annulus remains intact, with nanodiscs providing a snapshot of the lipid environment. Recent studies have provided evidence that phospholipids can exchange from the nanodiscs with either lipids at interfaces, or with other nanodiscs in bulk solution. Here we investigate kinetics of lipid exchange between three recently studied polymer-stabilized nanodiscs and supported lipid bilayers at the silicon-water interface. We show that lipid and polymer exchange occurs in all nanodiscs tested, although the rate and extent differs between different nanodisc types. Furthermore, we observe adsorption of nanodiscs to the supported lipid bilayer for one nanodisc system which used a polymer made using reversible addition-fragmentation chain transfer polymerization. These results have important implications in applications of polymer-stabilized nanodiscs, such as in the fabrication of solid-supported films containing membrane proteins.
Original languageEnglish
Pages (from-to)272-284
Number of pages13
JournalJournal of Colloid and Interface Science
Volume574
Early online date11 Apr 2020
DOIs
Publication statusPublished - 15 Aug 2020

Bibliographical note

Funding Information:
This work has been supported by an Engineering and Physical Sciences Research Council (EPSRC: EP/M506461/1 ) and Diamond Light Source studentship for SCLH and funding from the Biotechnology and Biological Sciences Research Council (BBSRC: BB/M018261/1 [TRD], BB/P009840/1 [TJK] and BB/L00335X/1 [TJK]). CT acknowledges STFC BioMemNet (studentship agreement #2990 ) and the University of Bath for PhD studentship funding.

Publisher Copyright:
© 2020

Keywords

  • Adsorption
  • Lipid exchange
  • Neutron reflectometry
  • Polymer-stabilized phospholipid nanodisc
  • Supported lipid bilayer
  • styrene maleic acid (SMA)
  • styrene-maleic acid lipid particle (SMALP)

ASJC Scopus subject areas

  • Electronic, Optical and Magnetic Materials
  • Biomaterials
  • Surfaces, Coatings and Films
  • Colloid and Surface Chemistry

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