Projects per year
Abstract
Understanding and controlling protein adsorption on surfaces is fundamental to many biological processes ranging from cell adhesion to the fabrication of protein biochips. In general, proteins need to retain their 3D conformation to perform their intended functions. However, when they are presented with a solid surface, complex interactions ranging from weak non- covalent binding to strong covalent bonding may occur, which can potentially induce conformational changes within the adsorbed protein. To investigate the surface adsorption process and its effects on a model protein, the chaperonin GroEL, we have applied contact mode atomic force microscopy, in buffer solution to probe the interactions between single proteins and surfaces in real space. We will discuss the adsorption of GroEL molecules on planar surfaces ( mica, graphite and gold) and specifically tailored nanostructured surfaces, which present structural features on the size scale of individual biological molecules.
Original language | English |
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Pages (from-to) | 353001- |
Journal | Journal of Physics: Condensed Matter |
Volume | 20 |
Issue number | 35 |
Early online date | 12 Aug 2008 |
DOIs | |
Publication status | Published - 3 Sept 2008 |
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Dive into the research topics of 'Adsorption of a model protein, the GroEL chaperonin, on surfaces'. Together they form a unique fingerprint.Projects
- 1 Finished
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Materials Program Platform Grant : Nanostructured Surfaces
Palmer, R., Guo, Q., Li, Z., Kaplan, A. & Robinson, A.
Engineering & Physical Science Research Council
1/07/07 → 31/12/11
Project: Research Councils