Adenosine monophosphate is elevated in the bronchoalveolar lavage fluid of mice with acute respiratory toxicity induced by nanoparticles with high surface hydrophobicity

Lea Ann Dailey, Raquel Hernández-Prieto, Ana Maria Casas-Ferreira, Marie-Christine Jones, Yanira Riffo-Vasquez, Encarnación Rodríguez-Gonzalo, Domenico Spina, Stuart A Jones, Norman W Smith, Ben Forbes, Clive Page, Cristina Legido-Quigley

Research output: Contribution to journalArticlepeer-review

11 Citations (Scopus)
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Abstract

Inhaled nanomaterials present a challenge to traditional methods and understanding of respiratory toxicology. In this study, a non-targeted metabolomics approach was used to investigate relationships between nanoparticle hydrophobicity, inflammatory outcomes and the metabolic fingerprint in bronchoalveolar fluid. Measures of acute lung toxicity were assessed following single-dose intratracheal administration of nanoparticles with varying surface hydrophobicity (i.e. pegylated lipid nanocapsules, polyvinyl acetate nanoparticles and polystyrene beads; listed in order of increasing hydrophobicity). Broncho-alveolar lavage (BAL) fluid was collected from mice exposed to nanoparticles at a surface area dose of 220 cm(2) and metabolite fingerprints were acquired via ultra pressure liquid chromatography-mass spectrometry-based metabolomics. Particles with high surface hydrophobicity were pro-inflammatory. Multivariate analysis of the resultant small molecule fingerprints revealed clear discrimination between the vehicle control and polystyrene beads (p 
Original languageEnglish
Pages (from-to)106-115
JournalNanotoxicology
Volume9
Issue number1
Early online date12 Mar 2014
DOIs
Publication statusPublished - 2015

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