Adapting in vitro dual perfusion of the human placenta to soluble oxygen tensions associated with normal and pre-eclamptic pregnancy

Fatimah Soydemir; Sitara Kuruvilla; Marie Brown; Warwick Dunn; Philip Day; Ian P Crocker; Philip N Baker; Colin P Sibley; Paul Brownbill

doi:10.1038/labinvest.2010.171

Adapting in vitro dual perfusion of the human placenta to soluble oxygen tensions associated with normal and pre-eclamptic pregnancy

Fatimah Soydemir, Sitara Kuruvilla, Marie Brown, Warwick Dunn, Philip Day, Ian P Crocker, Philip N Baker, Colin P Sibley, Paul Brownbill

Biosciences

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13 Citations (Scopus)

Abstract

For decades, superoxic ex vivo dual perfusion of the human placental lobule has been used as a model to study the physiology and metabolism of the placenta. The aim of this study was to further develop the technique to enable perfusion at soluble oxygen concentrations similar to those in normal pregnancy (normoxia) and in pre-eclampsia (PE; hypoxia). Our design involved reducing the mean soluble oxygen tension in the maternal-side intervillous space (IVS) perfusate to 5-7% and

Original language	English
Pages (from-to)	181-9
Number of pages	9
Journal	Laboratory investigation
Volume	91
Issue number	2
DOIs	https://doi.org/10.1038/labinvest.2010.171
Publication status	Published - Feb 2011

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AU - Crocker, Ian P

AU - Baker, Philip N

AU - Sibley, Colin P

AU - Brownbill, Paul

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Adapting in vitro dual perfusion of the human placenta to soluble oxygen tensions associated with normal and pre-eclamptic pregnancy

Abstract

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