Acrylate protects a marine bacterium from grazing by a ciliate predator

Zhao-Jie Teng; Peng Wang; Xiu-Lan Chen; Richard Guillonneau; Chun-Yang Li; Song-Bao Zou; Jun Gong; Kai-Wen Xu; Lin Han; Chao Wang; David j. Scanlan; Yin Chen; Yu-Zhong Zhang

doi:10.1038/s41564-021-00981-1

Acrylate protects a marine bacterium from grazing by a ciliate predator

Zhao-Jie Teng
, Peng Wang
, Xiu-Lan Chen
, Richard Guillonneau
, Chun-Yang Li
, Song-Bao Zou
, Jun Gong
, Kai-Wen Xu
, Lin Han
, Chao Wang
, David j. Scanlan
, Yin Chen
, Yu-Zhong Zhang^*

^*Corresponding author for this work

Biosciences

Research output: Contribution to journal › Article › peer-review

Abstract

Cleavage of dimethylsulfoniopropionate (DMSP) can deter herbivores in DMSP-producing eukaryotic algae; however, it is unclear whether a parallel defence mechanism operates in marine bacteria. Here we demonstrate that the marine bacterium Puniceibacterium antarcticum SM1211, which does not use DMSP as a carbon source, has a membrane-associated DMSP lyase, DddL. At high concentrations of DMSP, DddL causes an accumulation of acrylate around cells through the degradation of DMSP, which protects against predation by the marine ciliate Uronema marinum. The presence of acrylate can alter the grazing preference of U. marinum to other bacteria in the community, thereby influencing community structure.

Original language	English
Pages (from-to)	1351-1356
Number of pages	6
Journal	Nature Microbiology
Volume	6
Issue number	11
Early online date	25 Oct 2021
DOIs	https://doi.org/10.1038/s41564-021-00981-1
Publication status	Published - Nov 2021

Bibliographical note

Acknowledgements:
We thank Z.-F. Li and H.-Y. Yu from the State Key Laboratory of Microbial Technology at Shandong University for help and guidance in flow cytometry and laser-scanning confocal microscopy, respectively. We thank I. Hands-Portman and the Imaging Suite at the School of Life Sciences, University of Warwick for providing assistance with the use of confocal microscopy. This work was supported by the National Key Research and Development Program of China (grant no. 2018YFC1406700), the National Science Foundation of China (grant nos. 91851205, 31630012, U1706207, 31870052, 3217010695 and 41706152), the Fundamental Research Funds for the Central Universities (grant no. 202172002), Major Scientific and Technological Innovation Project (MSTIP) of Shandong Province (grant no. 2019JZZY010817) and the Program of Shandong for Taishan Scholars (tspd20181203).

Copyright:
© 2021. The Author(s), under exclusive licence to Springer Nature Limited.

Keywords

Acrylates/metabolism
Bacterial Proteins/genetics
Carbon-Sulfur Lyases/genetics
Ciliophora/microbiology
Rhodobacteraceae/enzymology
Seawater/microbiology
Sulfonium Compounds/metabolism

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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Cite this

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title = "Acrylate protects a marine bacterium from grazing by a ciliate predator",

abstract = "Cleavage of dimethylsulfoniopropionate (DMSP) can deter herbivores in DMSP-producing eukaryotic algae; however, it is unclear whether a parallel defence mechanism operates in marine bacteria. Here we demonstrate that the marine bacterium Puniceibacterium antarcticum SM1211, which does not use DMSP as a carbon source, has a membrane-associated DMSP lyase, DddL. At high concentrations of DMSP, DddL causes an accumulation of acrylate around cells through the degradation of DMSP, which protects against predation by the marine ciliate Uronema marinum. The presence of acrylate can alter the grazing preference of U. marinum to other bacteria in the community, thereby influencing community structure.",

keywords = "Acrylates/metabolism, Bacterial Proteins/genetics, Carbon-Sulfur Lyases/genetics, Ciliophora/microbiology, Rhodobacteraceae/enzymology, Seawater/microbiology, Sulfonium Compounds/metabolism",

author = "Zhao-Jie Teng and Peng Wang and Xiu-Lan Chen and Richard Guillonneau and Chun-Yang Li and Song-Bao Zou and Jun Gong and Kai-Wen Xu and Lin Han and Chao Wang and Scanlan, \{David j.\} and Yin Chen and Yu-Zhong Zhang",

note = "Acknowledgements: We thank Z.-F. Li and H.-Y. Yu from the State Key Laboratory of Microbial Technology at Shandong University for help and guidance in flow cytometry and laser-scanning confocal microscopy, respectively. We thank I. Hands-Portman and the Imaging Suite at the School of Life Sciences, University of Warwick for providing assistance with the use of confocal microscopy. This work was supported by the National Key Research and Development Program of China (grant no. 2018YFC1406700), the National Science Foundation of China (grant nos. 91851205, 31630012, U1706207, 31870052, 3217010695 and 41706152), the Fundamental Research Funds for the Central Universities (grant no. 202172002), Major Scientific and Technological Innovation Project (MSTIP) of Shandong Province (grant no. 2019JZZY010817) and the Program of Shandong for Taishan Scholars (tspd20181203). Copyright: {\textcopyright} 2021. The Author(s), under exclusive licence to Springer Nature Limited.",

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doi = "10.1038/s41564-021-00981-1",

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AU - Teng, Zhao-Jie

AU - Wang, Peng

AU - Chen, Xiu-Lan

AU - Guillonneau, Richard

AU - Li, Chun-Yang

AU - Zou, Song-Bao

AU - Gong, Jun

AU - Xu, Kai-Wen

AU - Han, Lin

AU - Wang, Chao

AU - Scanlan, David j.

AU - Chen, Yin

AU - Zhang, Yu-Zhong

N1 - Acknowledgements: We thank Z.-F. Li and H.-Y. Yu from the State Key Laboratory of Microbial Technology at Shandong University for help and guidance in flow cytometry and laser-scanning confocal microscopy, respectively. We thank I. Hands-Portman and the Imaging Suite at the School of Life Sciences, University of Warwick for providing assistance with the use of confocal microscopy. This work was supported by the National Key Research and Development Program of China (grant no. 2018YFC1406700), the National Science Foundation of China (grant nos. 91851205, 31630012, U1706207, 31870052, 3217010695 and 41706152), the Fundamental Research Funds for the Central Universities (grant no. 202172002), Major Scientific and Technological Innovation Project (MSTIP) of Shandong Province (grant no. 2019JZZY010817) and the Program of Shandong for Taishan Scholars (tspd20181203). Copyright: © 2021. The Author(s), under exclusive licence to Springer Nature Limited.

PY - 2021/11

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N2 - Cleavage of dimethylsulfoniopropionate (DMSP) can deter herbivores in DMSP-producing eukaryotic algae; however, it is unclear whether a parallel defence mechanism operates in marine bacteria. Here we demonstrate that the marine bacterium Puniceibacterium antarcticum SM1211, which does not use DMSP as a carbon source, has a membrane-associated DMSP lyase, DddL. At high concentrations of DMSP, DddL causes an accumulation of acrylate around cells through the degradation of DMSP, which protects against predation by the marine ciliate Uronema marinum. The presence of acrylate can alter the grazing preference of U. marinum to other bacteria in the community, thereby influencing community structure.

AB - Cleavage of dimethylsulfoniopropionate (DMSP) can deter herbivores in DMSP-producing eukaryotic algae; however, it is unclear whether a parallel defence mechanism operates in marine bacteria. Here we demonstrate that the marine bacterium Puniceibacterium antarcticum SM1211, which does not use DMSP as a carbon source, has a membrane-associated DMSP lyase, DddL. At high concentrations of DMSP, DddL causes an accumulation of acrylate around cells through the degradation of DMSP, which protects against predation by the marine ciliate Uronema marinum. The presence of acrylate can alter the grazing preference of U. marinum to other bacteria in the community, thereby influencing community structure.

KW - Acrylates/metabolism

KW - Bacterial Proteins/genetics

KW - Carbon-Sulfur Lyases/genetics

KW - Ciliophora/microbiology

KW - Rhodobacteraceae/enzymology

KW - Seawater/microbiology

KW - Sulfonium Compounds/metabolism

U2 - 10.1038/s41564-021-00981-1

DO - 10.1038/s41564-021-00981-1

M3 - Article

C2 - 34697458

SN - 2058-5276

VL - 6

SP - 1351

EP - 1356

JO - Nature Microbiology

JF - Nature Microbiology

IS - 11

ER -

Acrylate protects a marine bacterium from grazing by a ciliate predator

Abstract

Bibliographical note

Keywords

UN SDGs

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