Abstract
The synthesis of a novel modified nucleoside phosphoramidite, Acrylamide-dT-CE phosphoramidite, obtained in three steps from commercially available starting materials, is reported. It was readily incorporated into thrombin binding aptamer (TBA) sequences using automated solid-phase synthesis under ultra-mild conditions, with the modification shown not to adversely affect duplex stability, G-quadruplex structure, or thrombin binding. The reaction and integration of the modified strands with acrylamide polymers was evidenced by gel electrophoresis. The Acrylamide-dT functional handle promises to be an ideal synthon for preparing DNA–polymer hybrids for use in various macromolecular materials applications.
Original language | English |
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Pages (from-to) | 31511-31516 |
Number of pages | 6 |
Journal | RSC Advances |
Volume | 9 |
Issue number | 54 |
DOIs | |
Publication status | Published - 3 Oct 2019 |
ASJC Scopus subject areas
- General Chemistry
- General Chemical Engineering