Accumulation of polyhydroxybutyrate by a Serratia sp

HL Lugg, Rachel Sammons, Peter Marquis, Christopher Hewitt, Ping Yong, Marion Paterson-Beedle, Mark Redwood, Artemis Stamboulis, M Kashani, Michael Jenkins, Lynne Macaskie

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22 Citations (Scopus)
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Abstract

A strain of Serratia sp. showed intracellular electron-transparent inclusion bodies when incubated in the presence of citrate and glycerol 2-phosphate without nitrogen source following pre-growth under carbon-limitation in continuous culture. About 1.3 mmol citrate were consumed per 450 mg biomass, giving a calculated yield of maximally 55% of stored material per g of biomass dry wt. The inclusion bodies were stained with Sudan Black and Nile Red (NR), suggesting a lipid material, which was confirmed as polyhydroxybutyrate (PHB) by analysis of molecular fragments by GC and by FTIR spectroscopy of isolated bio-PHB in comparison with reference material. Multi-parameter flow cytometry in conjunction with NR fluorescence, and electron microscopy, showed that not all cells contained heavy PHB bodies, suggesting the potential for increasing the overall yield. The economic attractiveness is enhanced by the co-production of nanoscale hydroxyapatite (HA), a possible high-value precursor for bone replacement materials.
Original languageEnglish
Pages (from-to)481-491
Number of pages11
JournalBiotechnology Letters
Volume30
Issue number3
Early online date29 Dec 2007
DOIs
Publication statusPublished - Mar 2008

Keywords

  • Serratia sp.
  • fluorescence
  • flow cytometry
  • polyhydroxybutyrate

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