A two-stage, two-organism process for biohydrogen from glucose

MD Redwood, Lynne Macaskie

Research output: Contribution to journalArticlepeer-review

79 Citations (Scopus)
265 Downloads (Pure)


H2 can potentially be produced in a two-stage biological process: the fermentation of glucose by Escherichia coli HD701 and the photofermentation of the residual medium by Rhodobacter sphaeroides O.U. 001. In a typical batch fermentation, E. coli consumed glucose and produced H2, organic end-products and biomass. Organic end-products and residual glucose were removed during subsequent photofermentation by R. sphaeroides, with associated growth and neutralization of pH. However, photoproduction of H2 did not occur during photofermentation of the residual liquor per se due to the presence of fixed nitrogen compounds. Nevertheless, this two-stage approach could be applied to dispose of sugar-containing industrial wastes, H2 being used for on-site power generation.
Original languageEnglish
Pages (from-to)1514-1521
Number of pages8
JournalInternational Journal of Hydrogen Energy
Publication statusPublished - 1 Sept 2006


  • organic acids
  • Rhodobacter sphaeroides
  • biohydrogen
  • Escherichia coli
  • cross-feeding


Dive into the research topics of 'A two-stage, two-organism process for biohydrogen from glucose'. Together they form a unique fingerprint.

Cite this