A study of biofilm and non-line-of-sight bio-hydroxyapatite coatings using a Serratia sp.

P. Yong, M. Paterson-Beedle, W. Liu, Z. Zhang, D. A. Beauregard, M. L. Johns, L. E. Macaskie

Research output: Chapter in Book/Report/Conference proceedingConference contribution

2 Citations (Scopus)

Abstract

This study describes biofilm formation as a non line-of-sight coating method on support materials such as polyurethane foam, porous glass, polypropylene (PP) and titanium alloy, using a Serratia sp., which can manufacture extracellular nanoscale scaffolded hydroxyapatite (HA) crystals via enzymatic cleavage of glycerol 2-phosphate (G2P) in the presence of CaCl 2. Various microscopies and non-invasive magnetic resonance imaging were used to visualize the biofilm coating on the support surface. A novel micromanipulation technique was used to estimate the adhesive strength of native and HA-mineralized biofilms. The biofilm with HA was up to forty times stronger than that without HA. A coating of nano-HA (> 80 μm) onto a biofilm-Ti disc was achieved.

Original languageEnglish
Title of host publicationAdvanced Materials Research
Pages741-744
Number of pages4
Volume71-73
DOIs
Publication statusPublished - 2009
Event18th International Biohydrometallurgy Symposium, IBS 2009 - Bariloche, Argentina
Duration: 13 Sep 200917 Sep 2009

Publication series

NameAdvanced Materials Research
Volume71-73
ISSN (Print)10226680

Conference

Conference18th International Biohydrometallurgy Symposium, IBS 2009
Country/TerritoryArgentina
CityBariloche
Period13/09/0917/09/09

Keywords

  • Biofilm
  • Biomineralization
  • Hydroxyapatite
  • Serratia

ASJC Scopus subject areas

  • Engineering(all)

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