A single nucleotide polymorphism of interleukin-6 gene is related to plasma adrenomedullin levels

Hoi Kin Wong; Kwok Leung Ong; Raymond Y H Leung; Tai Hing Lam; Graham Neil Thomas; Karen S L Lam; Bernard M Y Cheung

doi:10.1111/cen.12078

A single nucleotide polymorphism of interleukin-6 gene is related to plasma adrenomedullin levels

Hoi Kin Wong, Kwok Leung Ong, Raymond Y H Leung, Tai Hing Lam, Graham Neil Thomas, Karen S L Lam, Bernard M Y Cheung

Research output: Contribution to journal › Article › peer-review

4 Citations (Scopus)

Abstract

OBJECTIVE: Elevated plasma adrenomedullin (ADM) levels are associated with cardiovascular diseases. Single nucleotide polymorphisms (SNPs) in the gene encoding ADM (ADM) are associated with plasma ADM levels. The presence of a nuclear factor for interleukin-6 (IL-6) expression binding site in the promoter region of the ADM gene suggests a possible relationship between the expression of the ADM and IL-6. Therefore, we investigated whether plasma ADM levels are related to SNPs in the gene encoding IL-6 (IL6).

METHODS: Plasma ADM levels were measured in 476 subjects in the Hong Kong Cardiovascular Risk Factor Prevalence Study-2 (CRISPS2). The subjects were genotyped for three tagging SNPs in the IL6 gene.

RESULTS: The minor allele frequencies of the IL6 SNPs rs17147230, rs1800796 and rs2069837 were 41·8%, 20·0% and 15·4%, respectively. The tagging SNP, rs17147230, was associated with plasma ADM levels after adjusting for age and sex (β=-0·096, P = 0·034). The association was significant in women (β=-0·115, P = 0·021) but not in men. Among all subjects, plasma ADM levels decreased with an increasing number of minor alleles of rs17147230 in multivariate analysis (P = 0·034). Compared to subjects with the AA genotype, subjects with the TT genotype had plasma ADM levels 12·8% lower (95% CI: 0·6-23·5%, P = 0·041). Haplotype analysis demonstrated a significant association of the haplotype ACA with plasma ADM levels in women (P < 0·05).

CONCLUSION: Plasma ADM levels are related to the SNP rs17147230 in IL6 gene. The effect of the polymorphism on inflammation and cardiovascular disease remains to be determined.

Original language	English
Pages (from-to)	504-9
Number of pages	6
Journal	Clinical Endocrinology
Volume	79
Issue number	4
DOIs	https://doi.org/10.1111/cen.12078
Publication status	Published - Oct 2013

Keywords

Adrenomedullin
Adult
Asian Continental Ancestry Group
Cardiovascular Diseases
Female
Gene Frequency
Genotype
Haplotypes
Hong Kong
Humans
Interleukin-6
Male
Middle Aged
Multivariate Analysis
Polymorphism, Single Nucleotide
Prevalence
Risk Factors
Sex Factors

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1111/cen.12078

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@article{fbd6c075d3b64adbb4e74b6cfa815835,

title = "A single nucleotide polymorphism of interleukin-6 gene is related to plasma adrenomedullin levels",

abstract = "OBJECTIVE: Elevated plasma adrenomedullin (ADM) levels are associated with cardiovascular diseases. Single nucleotide polymorphisms (SNPs) in the gene encoding ADM (ADM) are associated with plasma ADM levels. The presence of a nuclear factor for interleukin-6 (IL-6) expression binding site in the promoter region of the ADM gene suggests a possible relationship between the expression of the ADM and IL-6. Therefore, we investigated whether plasma ADM levels are related to SNPs in the gene encoding IL-6 (IL6).METHODS: Plasma ADM levels were measured in 476 subjects in the Hong Kong Cardiovascular Risk Factor Prevalence Study-2 (CRISPS2). The subjects were genotyped for three tagging SNPs in the IL6 gene.RESULTS: The minor allele frequencies of the IL6 SNPs rs17147230, rs1800796 and rs2069837 were 41·8%, 20·0% and 15·4%, respectively. The tagging SNP, rs17147230, was associated with plasma ADM levels after adjusting for age and sex (β=-0·096, P = 0·034). The association was significant in women (β=-0·115, P = 0·021) but not in men. Among all subjects, plasma ADM levels decreased with an increasing number of minor alleles of rs17147230 in multivariate analysis (P = 0·034). Compared to subjects with the AA genotype, subjects with the TT genotype had plasma ADM levels 12·8% lower (95% CI: 0·6-23·5%, P = 0·041). Haplotype analysis demonstrated a significant association of the haplotype ACA with plasma ADM levels in women (P < 0·05).CONCLUSION: Plasma ADM levels are related to the SNP rs17147230 in IL6 gene. The effect of the polymorphism on inflammation and cardiovascular disease remains to be determined.",

keywords = "Adrenomedullin, Adult, Asian Continental Ancestry Group, Cardiovascular Diseases, Female, Gene Frequency, Genotype, Haplotypes, Hong Kong, Humans, Interleukin-6, Male, Middle Aged, Multivariate Analysis, Polymorphism, Single Nucleotide, Prevalence, Risk Factors, Sex Factors",

author = "Wong, {Hoi Kin} and Ong, {Kwok Leung} and Leung, {Raymond Y H} and Lam, {Tai Hing} and Thomas, {Graham Neil} and Lam, {Karen S L} and Cheung, {Bernard M Y}",

note = "{\textcopyright} 2012 John Wiley & Sons Ltd.",

year = "2013",

month = oct,

doi = "10.1111/cen.12078",

language = "English",

volume = "79",

pages = "504--9",

journal = "Clinical Endocrinology",

issn = "0300-0664",

publisher = "Wiley",

number = "4",

}

TY - JOUR

T1 - A single nucleotide polymorphism of interleukin-6 gene is related to plasma adrenomedullin levels

AU - Wong, Hoi Kin

AU - Ong, Kwok Leung

AU - Leung, Raymond Y H

AU - Lam, Tai Hing

AU - Thomas, Graham Neil

AU - Lam, Karen S L

AU - Cheung, Bernard M Y

PY - 2013/10

Y1 - 2013/10

N2 - OBJECTIVE: Elevated plasma adrenomedullin (ADM) levels are associated with cardiovascular diseases. Single nucleotide polymorphisms (SNPs) in the gene encoding ADM (ADM) are associated with plasma ADM levels. The presence of a nuclear factor for interleukin-6 (IL-6) expression binding site in the promoter region of the ADM gene suggests a possible relationship between the expression of the ADM and IL-6. Therefore, we investigated whether plasma ADM levels are related to SNPs in the gene encoding IL-6 (IL6).METHODS: Plasma ADM levels were measured in 476 subjects in the Hong Kong Cardiovascular Risk Factor Prevalence Study-2 (CRISPS2). The subjects were genotyped for three tagging SNPs in the IL6 gene.RESULTS: The minor allele frequencies of the IL6 SNPs rs17147230, rs1800796 and rs2069837 were 41·8%, 20·0% and 15·4%, respectively. The tagging SNP, rs17147230, was associated with plasma ADM levels after adjusting for age and sex (β=-0·096, P = 0·034). The association was significant in women (β=-0·115, P = 0·021) but not in men. Among all subjects, plasma ADM levels decreased with an increasing number of minor alleles of rs17147230 in multivariate analysis (P = 0·034). Compared to subjects with the AA genotype, subjects with the TT genotype had plasma ADM levels 12·8% lower (95% CI: 0·6-23·5%, P = 0·041). Haplotype analysis demonstrated a significant association of the haplotype ACA with plasma ADM levels in women (P < 0·05).CONCLUSION: Plasma ADM levels are related to the SNP rs17147230 in IL6 gene. The effect of the polymorphism on inflammation and cardiovascular disease remains to be determined.

AB - OBJECTIVE: Elevated plasma adrenomedullin (ADM) levels are associated with cardiovascular diseases. Single nucleotide polymorphisms (SNPs) in the gene encoding ADM (ADM) are associated with plasma ADM levels. The presence of a nuclear factor for interleukin-6 (IL-6) expression binding site in the promoter region of the ADM gene suggests a possible relationship between the expression of the ADM and IL-6. Therefore, we investigated whether plasma ADM levels are related to SNPs in the gene encoding IL-6 (IL6).METHODS: Plasma ADM levels were measured in 476 subjects in the Hong Kong Cardiovascular Risk Factor Prevalence Study-2 (CRISPS2). The subjects were genotyped for three tagging SNPs in the IL6 gene.RESULTS: The minor allele frequencies of the IL6 SNPs rs17147230, rs1800796 and rs2069837 were 41·8%, 20·0% and 15·4%, respectively. The tagging SNP, rs17147230, was associated with plasma ADM levels after adjusting for age and sex (β=-0·096, P = 0·034). The association was significant in women (β=-0·115, P = 0·021) but not in men. Among all subjects, plasma ADM levels decreased with an increasing number of minor alleles of rs17147230 in multivariate analysis (P = 0·034). Compared to subjects with the AA genotype, subjects with the TT genotype had plasma ADM levels 12·8% lower (95% CI: 0·6-23·5%, P = 0·041). Haplotype analysis demonstrated a significant association of the haplotype ACA with plasma ADM levels in women (P < 0·05).CONCLUSION: Plasma ADM levels are related to the SNP rs17147230 in IL6 gene. The effect of the polymorphism on inflammation and cardiovascular disease remains to be determined.

KW - Adrenomedullin

KW - Adult

KW - Asian Continental Ancestry Group

KW - Cardiovascular Diseases

KW - Female

KW - Gene Frequency

KW - Genotype

KW - Haplotypes

KW - Hong Kong

KW - Humans

KW - Interleukin-6

KW - Male

KW - Middle Aged

KW - Multivariate Analysis

KW - Polymorphism, Single Nucleotide

KW - Prevalence

KW - Risk Factors

KW - Sex Factors

U2 - 10.1111/cen.12078

DO - 10.1111/cen.12078

M3 - Article

C2 - 23088295

SN - 0300-0664

VL - 79

SP - 504

EP - 509

JO - Clinical Endocrinology

JF - Clinical Endocrinology

IS - 4

ER -

A single nucleotide polymorphism of interleukin-6 gene is related to plasma adrenomedullin levels

Abstract

Keywords

UN SDGs

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