A single aromatic residue in transcriptional repressor protein KorA is critical for cooperativity with its co-regulator KorB.

Lewis Bingle, KV Rajasekar, S Muntaha, V Nadella, Eva Hyde, Christopher Thomas

Research output: Contribution to journalArticlepeer-review

11 Citations (Scopus)
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Abstract

A central feature of broad host range IncP-1 plasmids is the set of regulatory circuits that tightly control plasmid core functions under steady-state conditions. Cooperativity between KorB and either KorA or TrbA repressor proteins is a key element of these circuits and deletion analysis has implicated the conserved C-terminal domain of KorAand TrbAin this interaction. By NMR we show that KorA and KorB interact directly and identify KorA amino acids that are affected on KorB binding. Studies on mutants showed that tyrosine 84 (or phenylalanine, in some alleles) is dispensable for repressor activity but critical for the specific interaction with KorB in both in vivo reporter gene assays and in vitro electrophoretic mobility shift and co-purification assays. This confirms that direct and specific protein–protein interactions are responsible for the cooperativity observed between KorB and its corepressors and lays the basis for determining the biological importance of this cooperativity.
Original languageEnglish
Pages (from-to)1502-14
Number of pages13
JournalMolecular Microbiology
Volume70
Issue number6
DOIs
Publication statusPublished - 1 Dec 2008

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