A review of co-culture models to study the oral microenvironment and disease

Sophie Mountcastle, Sophie Cox, Rachel Sammons, Sara Jabbari, Richard Shelton, Sarah Kuehne

Research output: Contribution to journalArticlepeer-review

2 Citations (Scopus)
226 Downloads (Pure)


Co-cultures allow for the study of cell–cell interactions between different eukaryotic species or with bacteria. Such an approach has enabled researchers to more closely mimic complex tissue structures. This review is focused on co-culture systems modelling the oral cavity, which have been used to evaluate this unique cellular environment and understand disease progression. Over time, these systems have developed significantly from simple 2D eukaryotic cultures and planktonic bacteria to more complex 3D tissue engineered structures and biofilms. Careful selection and design of the co-culture along with critical parameters, such as seeding density and choice of analysis method, have resulted in several advances. This review provides a comparison of existing co-culture systems for the oral environment, with emphasis on progression of 3D models and the opportunity to harness techniques from other fields to improve current methods. While filling a gap in navigating this literature, this review ultimately supports the development of this vital technique in the field of oral biology.
Original languageEnglish
Article number1773122
JournalJournal of Oral Microbiology
Issue number1
Publication statusPublished - 4 Jun 2020


  • 3D-in vitro models
  • Co-culture
  • NC3Rs
  • biofilms
  • oral cavity
  • oral disease

ASJC Scopus subject areas

  • Dentistry (miscellaneous)
  • Microbiology (medical)
  • Infectious Diseases


Dive into the research topics of 'A review of co-culture models to study the oral microenvironment and disease'. Together they form a unique fingerprint.

Cite this