A purified plasma membrane fraction isolated from rat liver under isotonic conditions.

R Coleman; RH Michell; JB Finean; JN Hawthorne

doi:10.1016/0005-2736(67)90089-2

A purified plasma membrane fraction isolated from rat liver under isotonic conditions.

R Coleman
, RH Michell
, JB Finean
, JN Hawthorne

Biosciences

Research output: Contribution to journal › Article › peer-review

136 Citations (Scopus)

Abstract

A fraction rich in plasma membrane has been isolated from perfused rat liver. The purity of the preparation is comparable with that of material isolated by other procedures and the yield of the purified fraction is appreciably greater. Isotonic sucrose is used in the early stages of the cell fractionation and it is therefore possible to isolate both a plasma membrane fraction and other cell components from the same homogenate.

Original language	English
Pages (from-to)	573-579
Journal	Biochimica et Biophysica Acta
Volume	135
Issue number	4
DOIs	https://doi.org/10.1016/0005-2736(67)90089-2
Publication status	Published - Sept 1967

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10.1016/0005-2736(67)90089-2

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title = "A purified plasma membrane fraction isolated from rat liver under isotonic conditions.",

abstract = "A fraction rich in plasma membrane has been isolated from perfused rat liver. The purity of the preparation is comparable with that of material isolated by other procedures and the yield of the purified fraction is appreciably greater. Isotonic sucrose is used in the early stages of the cell fractionation and it is therefore possible to isolate both a plasma membrane fraction and other cell components from the same homogenate.",

author = "R Coleman and RH Michell and JB Finean and JN Hawthorne",

year = "1967",

month = sep,

doi = "10.1016/0005-2736(67)90089-2",

language = "English",

volume = "135",

pages = "573--579",

journal = "Biochimica et Biophysica Acta",

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T1 - A purified plasma membrane fraction isolated from rat liver under isotonic conditions.

AU - Coleman, R

AU - Michell, RH

AU - Finean, JB

AU - Hawthorne, JN

PY - 1967/9

Y1 - 1967/9

N2 - A fraction rich in plasma membrane has been isolated from perfused rat liver. The purity of the preparation is comparable with that of material isolated by other procedures and the yield of the purified fraction is appreciably greater. Isotonic sucrose is used in the early stages of the cell fractionation and it is therefore possible to isolate both a plasma membrane fraction and other cell components from the same homogenate.

AB - A fraction rich in plasma membrane has been isolated from perfused rat liver. The purity of the preparation is comparable with that of material isolated by other procedures and the yield of the purified fraction is appreciably greater. Isotonic sucrose is used in the early stages of the cell fractionation and it is therefore possible to isolate both a plasma membrane fraction and other cell components from the same homogenate.

UR - http://europepmc.org/abstract/med/4292717

U2 - 10.1016/0005-2736(67)90089-2

DO - 10.1016/0005-2736(67)90089-2

M3 - Article

C2 - 4292717

SN - 1874-9399

VL - 135

SP - 573

EP - 579

JO - Biochimica et Biophysica Acta

JF - Biochimica et Biophysica Acta

IS - 4

ER -

A purified plasma membrane fraction isolated from rat liver under isotonic conditions.

Abstract

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