A purified plasma membrane fraction isolated from rat liver under isotonic conditions.

R Coleman, RH Michell, JB Finean, JN Hawthorne

Research output: Contribution to journalArticlepeer-review

136 Citations (Scopus)

Abstract

A fraction rich in plasma membrane has been isolated from perfused rat liver. The purity of the preparation is comparable with that of material isolated by other procedures and the yield of the purified fraction is appreciably greater. Isotonic sucrose is used in the early stages of the cell fractionation and it is therefore possible to isolate both a plasma membrane fraction and other cell components from the same homogenate.
Original languageEnglish
Pages (from-to)573-579
JournalBiochimica et Biophysica Acta
Volume135
Issue number4
DOIs
Publication statusPublished - Sep 1967

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