A plant signal sequence enhances the secretion of bacterial ChiA in transgenic tobacco

P Lund, P Dunsmuir

Research output: Contribution to journalArticlepeer-review

23 Citations (Scopus)

Abstract

When the secreted bacterial protein ChiA is expressed in transgenic tobacco, a fraction of the protein is glycosylated and secreted from the plant cells; however most of the protein remains inside the cells. We tested whether the efficiency of secretion could be improved by replacing the bacterial signal sequence with a plant signal sequence. We found the signal sequence and the first two amino acids of the PR1b protein attached to the ChiA mature protein directs complete glycosylation and secretion of the ChiA from plant cells. Glycosylation of this protein is not required for its efficient secretion from plant cells.
Original languageEnglish
Pages (from-to)47-53
Number of pages7
JournalPlant Molecular Biology
Volume18
Issue number1
Publication statusPublished - Jan 1992

Keywords

  • Plants, Toxic
  • Immunoblotting
  • Chitinase
  • Bacterial Proteins
  • Plants, Genetically Modified
  • Tobacco
  • Molecular Sequence Data
  • Recombinant Fusion Proteins
  • Amino Acid Sequence
  • Protein Sorting Signals
  • Glycosylation

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