Abstract
When the secreted bacterial protein ChiA is expressed in transgenic tobacco, a fraction of the protein is glycosylated and secreted from the plant cells; however most of the protein remains inside the cells. We tested whether the efficiency of secretion could be improved by replacing the bacterial signal sequence with a plant signal sequence. We found the signal sequence and the first two amino acids of the PR1b protein attached to the ChiA mature protein directs complete glycosylation and secretion of the ChiA from plant cells. Glycosylation of this protein is not required for its efficient secretion from plant cells.
Original language | English |
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Pages (from-to) | 47-53 |
Number of pages | 7 |
Journal | Plant Molecular Biology |
Volume | 18 |
Issue number | 1 |
Publication status | Published - Jan 1992 |
Keywords
- Plants, Toxic
- Immunoblotting
- Chitinase
- Bacterial Proteins
- Plants, Genetically Modified
- Tobacco
- Molecular Sequence Data
- Recombinant Fusion Proteins
- Amino Acid Sequence
- Protein Sorting Signals
- Glycosylation