A non-canonical promoter element drives spurious transcription of horizontally acquired bacterial genes

Emily A Warman, Shivani S Singh, Alicia G Gubieda, David C Grainger

Research output: Contribution to journalArticlepeer-review

3 Citations (Scopus)
95 Downloads (Pure)

Abstract

RNA polymerases initiate transcription at DNA sequences called promoters. In bacteria, the best conserved promoter feature is the AT-rich -10 element; a sequence essential for DNA unwinding. Further elements, and gene regulatory proteins, are needed to recruit RNA polymerase to the -10 sequence. Hence, -10 elements cannot function in isolation. Many horizontally acquired genes also have a high AT-content. Consequently, sequences that resemble the -10 element occur frequently. As a result, foreign genes are predisposed to spurious transcription. However, it is not clear how RNA polymerase initially recognizes such sequences. Here, we identify a non-canonical promoter element that plays a key role. The sequence, itself a short AT-tract, resides 5 base pairs upstream of otherwise cryptic -10 elements. The AT-tract alters DNA conformation and enhances contacts between the DNA backbone and RNA polymerase.
Original languageEnglish
Pages (from-to)4891-4901
Number of pages11
JournalNucleic Acids Research
Volume48
Issue number9
Early online date16 Apr 2020
DOIs
Publication statusPublished - 21 May 2020

Bibliographical note

© The Author(s) 2020. Published by Oxford University Press on behalf of Nucleic Acids Research.

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