A neuraminidase from Streptococcus pneumoniae has the features of a surface protein

M Cámara, G J Boulnois, P W Andrew, T J Mitchell

Research output: Contribution to journalArticlepeer-review

118 Citations (Scopus)


A gene from Streptococcus pneumoniae (nanA), with features entirely consistent with a neuraminidase gene, has been sequenced. High levels of neuraminidase activity were obtained after cloning of this gene, without flanking sequences, into a high-expression vector. RNA hybridization studies have shown that the gene is transcribed by a virulent pneumococcus strain. The predicted molecular weight of the protein and certain amino acid sequences are typical of other neuraminidases. NanA contains the four copies of the sequence SXDXGXTW that is present in all the bacterial neuraminidases previously described. Kyte and Doolittle analysis showed that NanA is a hydrophilic protein with hydrophobic domains at the N terminus and the C terminus. A putative signal peptide was found in the N terminus of this protein, indicating that the protein is exported from the pneumococcus. The C terminus has the features of the anchor motif found in other surface proteins from gram-positive bacteria. Electron microscopy studies showed the presence of neuraminidase associated with the cell surface of the pneumococcus.

Original languageEnglish
Pages (from-to)3688-95
Number of pages8
JournalInfection and Immunity
Issue number9
Publication statusPublished - Sept 1994


  • Amino Acid Sequence
  • Base Sequence
  • Membrane Proteins
  • Molecular Sequence Data
  • Neuraminidase
  • Open Reading Frames
  • RNA, Messenger
  • Streptococcus pneumoniae


Dive into the research topics of 'A neuraminidase from Streptococcus pneumoniae has the features of a surface protein'. Together they form a unique fingerprint.

Cite this