A modified protocol for the isolation of Primary Human Hepatocytes with improved viability and function from normal and diseased human liver

David Bartlett; Philip Newsome

doi:10.1007/978-1-4939-6506-9_4

A modified protocol for the isolation of Primary Human Hepatocytes with improved viability and function from normal and diseased human liver

David Bartlett
, Philip Newsome

Immunology and Immunotherapy

Research output: Chapter in Book/Report/Conference proceeding › Chapter

1 Citation (Scopus)

Abstract

Successful hepatocyte isolation is critical for continued development of cellular transplantation. However, most tissue available for research is from diseased liver and the results of hepatocyte isolation from such tissue are inferior compared to normal tissue. Here we describe a modified method, combining the use of Liberase and N-acetylcysteine (NAC), for the isolation of primary human hepatocytes with high viability from normal and diseased liver.

Original language	English
Title of host publication	Hepatocyte Transplantation
Subtitle of host publication	Methods and Protocols
Place of Publication	New York
Publisher	Springer
Pages	61-73
Volume	1506
ISBN (Electronic)	978-1-4939-6506-9
ISBN (Print)	978-1-4939-6504-5
DOIs	https://doi.org/10.1007/978-1-4939-6506-9_4
Publication status	Published - 10 Nov 2017

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

SDG 3 Good Health and Well-being

Keywords

Human hepatocyte
Hepatocyte isolation
Hepatocyte viability
Liberase N-Acetylcysteine

Access to Document

10.1007/978-1-4939-6506-9_4

Cite this

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AB - Successful hepatocyte isolation is critical for continued development of cellular transplantation. However, most tissue available for research is from diseased liver and the results of hepatocyte isolation from such tissue are inferior compared to normal tissue. Here we describe a modified method, combining the use of Liberase and N-acetylcysteine (NAC), for the isolation of primary human hepatocytes with high viability from normal and diseased liver.

KW - Human hepatocyte

KW - Hepatocyte isolation

KW - Hepatocyte viability

KW - Liberase N-Acetylcysteine

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M3 - Chapter

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BT - Hepatocyte Transplantation

PB - Springer

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ER -

A modified protocol for the isolation of Primary Human Hepatocytes with improved viability and function from normal and diseased human liver

Abstract

UN SDGs

Keywords

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