A method for zebrafish follicle transplantation into recipient mothers for the generation of fertilizable eggs and viable offspring

Zsolt Csenki, Ferenc Mueller

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

In zebrafish and other externally developing animals, maternal effect gene products significantly contribute to embryonic and larval development. Several methods exist for both forward and reverse genetic manipulation of maternal gene products, yet it remains technically difficult to interfere with maternal gene products. Therefore, alternative methods to manipulate maternal factors in oocytes remain of interest. Here we describe a method which allows manipulation and subsequent transplantation of stage I–II follicles of zebrafish into recipient mothers where donor oocytes can develop into mature eggs and produce viable offspring. Additionally, we describe a simple microinjection protocol for injecting reporter constructs into follicles as a proxy for manipulating maternal effect genes.

Original languageEnglish
Title of host publicationMethods in Molecular Biology
PublisherHumana Press
Pages343-352
Number of pages10
DOIs
Publication statusPublished - 2019

Publication series

NameMethods in Molecular Biology
Volume1920
ISSN (Print)1064-3745

Bibliographical note

Publisher Copyright:
© Springer Science+Business Media, LLC, part of Springer Nature 2019.

Keywords

  • Follicle
  • Maternal effect
  • Oogenesis
  • Transgenic
  • Transplantation

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

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