A method for generating marker-less gene deletions in multidrug-resistant Acinetobacter baumannii

Ismawati Mohammad Amin, Grace E Richmond, Paromita Sen, Tse Hsien Koh, Laura J V Piddock, Kim Lee Chua

Research output: Contribution to journalArticlepeer-review

40 Citations (Scopus)
196 Downloads (Pure)

Abstract

Acinetobacter baumannii is an important nosocomial pathogen that has become increasingly resistant to multiple antibiotics. Genetic manipulation of MDR A. baumannii is useful especially for defining the contribution of each active efflux mechanism in multidrug resistance. Existing methods rely on the use of an antibiotic selection marker and are not suited for multiple gene deletions.
Original languageEnglish
Article number158
JournalBMC Microbiology
Volume13
DOIs
Publication statusPublished - 13 Jul 2013

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