A dataset of dual calcium and voltage optical mapping in healthy and hypertrophied murine hearts

Shicheng He, Kun Kou, Christopher O’shea, Tangting Chen, Razik Mu-u-min, Ruirui Dong, Huiying Ren, Xiaolin Zhou, Zhongcai Fan, Xiaoqiu Tan, Davor Pavlovic, Xianhong Ou, Ming Lei

Research output: Contribution to journalArticlepeer-review

Abstract

Pathological hypertrophy underlies sudden cardiac death due to its high incidence of occurrence of ventricular arrhythmias. The alteration of transmural electrophysiological properties in hypertrophic cardiac murine tissue has never been explored previously. In this dataset, we have for the first time conducted high-throughput simultaneous optical imaging of transmembrane potential and calcium transients (CaT) throughout the entire hypertrophic murine hearts at high temporal and spatial resolution. Using ElectroMap, we have conducted multiple parameters analysis including action potential duration/calcium transient duration, conduction velocity, alternans and diastolic interval. Voltage-calcium latency was measured as time difference between action potential and CaT peak. The dataset therefore provides the first high spatial resolution transmural electrophysiological profiling of the murine heart, allowing interrogation of mechanisms driving ventricular arrhythmias associated with pathological hypertrophy. The dataset allows for further reuse and detailed analyses of geometrical, topological and functional analyses and reconstruction of 2-dimensional and 3-dimentional models.
Original languageEnglish
Article number314
Number of pages9
JournalScientific Data
Volume8
Issue number1
Early online date16 Dec 2021
DOIs
Publication statusPublished - Dec 2021

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