A compact, multidimensional spectrofluorometer exploiting supercontinuum generation

Hugh B. Manning; Gordon T. Kennedy; Dylan Owen; David M. Grant; Anthony I. Magee; Mark A. A. Neil; Yoshifumi Itoh; Chris Dunsby; Paul M. W. French

doi:10.1002/jbio.200810051

A compact, multidimensional spectrofluorometer exploiting supercontinuum generation

Hugh B. Manning, Gordon T. Kennedy, Dylan Owen, David M. Grant, Anthony I. Magee, Mark A. A. Neil, Yoshifumi Itoh, Chris Dunsby, Paul M. W. French

Immunology and Immunotherapy

Research output: Contribution to journal › Article › peer-review

34 Citations (Scopus)

Abstract

We report a novel, compact and automated multidimensioual spectrofluorometer that exploits a fibre-laser-pumped ultrafast supercontinuum source to provide resolution with respect to intensity, excitation and emission wavelength, decay time and polarisation. This instrument has been applied to study the photophysics of the phase-sensitive membrane probe di-4-ANEPPDHQ and to characterise protein-protein interactions via Forster resonance energy transfer. It can be applied to in situ measurements via a fibre-optic probe in medical and other contexts and is demonstrated here to provide a comprehensive characterisation of tissue autofluorescence.

Original language	English
Pages (from-to)	494-505
Number of pages	12
Journal	Journal of Biophotonics
Volume	1
Issue number	6
Early online date	1 Dec 2008
DOIs	https://doi.org/10.1002/jbio.200810051
Publication status	E-pub ahead of print - 1 Dec 2008

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title = "A compact, multidimensional spectrofluorometer exploiting supercontinuum generation",

abstract = "We report a novel, compact and automated multidimensioual spectrofluorometer that exploits a fibre-laser-pumped ultrafast supercontinuum source to provide resolution with respect to intensity, excitation and emission wavelength, decay time and polarisation. This instrument has been applied to study the photophysics of the phase-sensitive membrane probe di-4-ANEPPDHQ and to characterise protein-protein interactions via Forster resonance energy transfer. It can be applied to in situ measurements via a fibre-optic probe in medical and other contexts and is demonstrated here to provide a comprehensive characterisation of tissue autofluorescence.",

author = "Manning, {Hugh B.} and Kennedy, {Gordon T.} and Dylan Owen and Grant, {David M.} and Magee, {Anthony I.} and Neil, {Mark A. A.} and Yoshifumi Itoh and Chris Dunsby and French, {Paul M. W.}",

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T1 - A compact, multidimensional spectrofluorometer exploiting supercontinuum generation

AU - Manning, Hugh B.

AU - Kennedy, Gordon T.

AU - Owen, Dylan

AU - Grant, David M.

AU - Magee, Anthony I.

AU - Neil, Mark A. A.

AU - Itoh, Yoshifumi

AU - Dunsby, Chris

AU - French, Paul M. W.

PY - 2008/12/1

Y1 - 2008/12/1

N2 - We report a novel, compact and automated multidimensioual spectrofluorometer that exploits a fibre-laser-pumped ultrafast supercontinuum source to provide resolution with respect to intensity, excitation and emission wavelength, decay time and polarisation. This instrument has been applied to study the photophysics of the phase-sensitive membrane probe di-4-ANEPPDHQ and to characterise protein-protein interactions via Forster resonance energy transfer. It can be applied to in situ measurements via a fibre-optic probe in medical and other contexts and is demonstrated here to provide a comprehensive characterisation of tissue autofluorescence.

AB - We report a novel, compact and automated multidimensioual spectrofluorometer that exploits a fibre-laser-pumped ultrafast supercontinuum source to provide resolution with respect to intensity, excitation and emission wavelength, decay time and polarisation. This instrument has been applied to study the photophysics of the phase-sensitive membrane probe di-4-ANEPPDHQ and to characterise protein-protein interactions via Forster resonance energy transfer. It can be applied to in situ measurements via a fibre-optic probe in medical and other contexts and is demonstrated here to provide a comprehensive characterisation of tissue autofluorescence.

U2 - 10.1002/jbio.200810051

DO - 10.1002/jbio.200810051

M3 - Article

SN - 1864-063X

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SP - 494

EP - 505

JO - Journal of Biophotonics

JF - Journal of Biophotonics

IS - 6

ER -

A compact, multidimensional spectrofluorometer exploiting supercontinuum generation

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