A cell cycle-coordinated Polymerase II transcription compartment encompasses gene expression before global genome activation

Yavor Hadzhiev, Haseeb K Qureshi, Lucy Wheatley, Ledean Cooper, Aleksandra Jasiulewicz, Huy Van Nguyen, Joseph W Wragg, Divyasree Poovathumkadavil, Sascha Conic, Sarah Bajan, Attila Sik, György Hutvàgner, Làszlò Tora, Agnieszka Gambus, John S Fossey, Ferenc Müller

Research output: Contribution to journalArticlepeer-review

16 Citations (Scopus)
175 Downloads (Pure)

Abstract

Most metazoan embryos commence development with rapid, transcriptionally silent cell divisions, with genome activation delayed until the mid-blastula transition (MBT). However, a set of genes escapes global repression and gets activated before MBT. Here we describe the formation and the spatio-temporal dynamics of a pair of distinct transcription compartments, which encompasses the earliest gene expression in zebrafish. 4D imaging of pri-miR430 and zinc-finger-gene activities by a novel, native transcription imaging approach reveals transcriptional sharing of nuclear compartments, which are regulated by homologous chromosome organisation. These compartments carry the majority of nascent-RNAs and active Polymerase II, are chromatin-depleted and represent the main sites of detectable transcription before MBT. Transcription occurs during the S-phase of increasingly permissive cleavage cycles. It is proposed, that the transcription compartment is part of the regulatory architecture of embryonic nuclei and offers a transcriptionally competent environment to facilitate early escape from repression before global genome activation.

Original languageEnglish
Article number691
Pages (from-to)691
JournalNature Communications
Volume10
Issue number1
DOIs
Publication statusPublished - 11 Feb 2019

ASJC Scopus subject areas

  • Chemistry(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Physics and Astronomy(all)

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