Research output per year
Research output per year
- 25 results
Search results
-
2022
High quality genome annotation and expression visualisation of a mupirocin-producing bacterium
Haines, A. S., Kendrew, S. G., Crowhurst, N., Stephens, E. R., Connolly, J., Hothersall, J., Miller, C. E., Collis, A. J., Huckle, B. D. & Thomas, C. M., 5 May 2022, In: PLoS ONE. 17, 5, e0268072.Research output: Contribution to journal › Article › peer-review
Open AccessFile70 Downloads (Pure) -
Inexpensive protein overexpression driven by the NarL transcription activator protein
Hothersall, J., Lai, S., Zhang, N., Godfrey, R., Ruanto, P., Bischoff, S., Robinson, C., Overton, T., Busby, S. & Browning, D., 13 May 2022, In: Biotechnology and Bioengineering. 119, 6, p. 1614-1623 10 p.Research output: Contribution to journal › Article › peer-review
Open AccessFile96 Downloads (Pure) -
New vectors for urea-inducible recombinant protein production
Hothersall, J., Osgerby, A., Godfrey, R., Overton, T., Busby, S. & Browning, D., 25 Dec 2022, In: New Biotechnology. 72, p. 89-96 8 p.Research output: Contribution to journal › Article › peer-review
Open AccessFile68 Downloads (Pure) -
2021
The PAR promoter expression system: modified lac promoters for controlled recombinant protein production in Escherichia coli
Hothersall, J., Godfrey, R., Fanitsios, C., Overton, T., Busby, S. & Browning, D., 25 Sept 2021, In: New Biotechnology. 64, p. 1-8 8 p.Research output: Contribution to journal › Article › peer-review
Open AccessFile645 Downloads (Pure) -
2019
Defining the genes for the final steps in biosynthesis of the complex polyketide antibiotic mupirocin by Pseudomonas fluorescens NCIMB10586
Connolly, J., Wilson, A., Macioszek, M., Song, Z., Wang, L., Mohammad, H., Yadav, M., di Martino, M., Litchfield, C., Hothersall, J., Haines, A., Stephens, E., Crump, M. P., Willis, C. L., Simpson, T. J., Winn, P. & Thomas, C., May 2019, In: Scientific Reports. 9, 1, 1542.Research output: Contribution to journal › Article › peer-review
Open AccessFile3 Citations (Scopus)190 Downloads (Pure) -
2018
Fine tuning of antibiotic activity by a tailoring hydroxylase in a trans-AT polyketide synthase pathway
Mohammad, H., Connolly, J., Song, Z., Hothersall, J., Race, P. R., Willis, C. L., Simpson, T. J., Winn, P. & Thomas, C., 24 Jan 2018, (E-pub ahead of print) In: ChemBioChem. 5 p., cbic.201800036.Research output: Contribution to journal › Article › peer-review
Open AccessFile252 Downloads (Pure) -
2017
Selected mutations reveal new intermediates in the biosynthesis of mupirocin and the thiomarinol antibiotics
Gao, S-S., Wang, L., Song, Z., Hothersall, J., Stevens, E. R., Connolly, J., Winn, P. J., Cox, R. J., Crump, M. P., Race, P. R., Thomas, C., Simpson, T. J. & Willis, C. L., 9 Feb 2017, (E-pub ahead of print) In: Angewandte Chemie (International Edition) . 56, 14, p. 3930-3934Research output: Contribution to journal › Article › peer-review
Open AccessFile5 Citations (Scopus)375 Downloads (Pure) -
2014
Biosynthesis of mupirocin by pseudomonas fluorescens NCIMB 10586 involves parallel pathways
Gao, S. S., Hothersall, J., Wu, JE., Murphy, A. C., Song, Z., Stephens, E. R., Thomas, C. M., Crump, M. P., Cox, R. J., Simpson, T. J. & Willis, C. L., 9 Apr 2014, In: Journal of the American Chemical Society. 136, 14, p. 5501-5507 7 p.Research output: Contribution to journal › Article › peer-review
20 Citations (Scopus) -
Biosynthesis of thiomarinol A and related metabolites of Pseudoalteromonas sp. SANK 73390
Murphy, A. C., Gao, S. S., Han, L. C., Carobene, S., Fukuda, D., Song, Z., Hothersall, J., Cox, R. J., Crosby, J., Crump, M. P., Willis, C. L., Simpson, T. J. & Thomas, C., Jan 2014, In: Chemical Science. 5, 1, p. 397-402 6 p.Research output: Contribution to journal › Article › peer-review
26 Citations (Scopus) -
2013
A conserved motif flags acyl carrier proteins for β-branching in polyketide synthesis
Haines, A. S., Dong, X., Song, Z., Farmer, R., Williams, C., Hothersall, J., Płoskoń, E., Wattana-amorn, P., Stephens, E. R., Yamada, E., Gurney, R., Takebayashi, Y., Masschelein, J., Cox, R. J., Lavigne, R., Willis, C. L., Simpson, T. J., Crosby, J., Winn, P. J., Thomas, C. M., & 1 others, Nov 2013, In: Nature Chemical Biology. 9, 11, p. 685-692 8 p.Research output: Contribution to journal › Article › peer-review
39 Citations (Scopus) -
2011
A Natural Plasmid Uniquely Encodes Two Biosynthetic Pathways Creating a Potent Anti-MRSA Antibiotic.
Fukuda, D., Haines, A., Song, Z., Murphy, AC., Hothersall, J., Stephens, E., Gurney, R., Cox, RJ., Crosby, J., Willis, CL., Simpson, TJ. & Thomas, C., 1 Jan 2011, In: PLoS ONE. 6, 3, p. e18031Research output: Contribution to journal › Article › peer-review
Open AccessFile45 Citations (Scopus)309 Downloads (Pure) -
Engineered Thiomarinol Antibiotics Active against MRSA Are Generated by Mutagenesis and Mutasynthesis of Pseudoalteromonas SANK73390
Murphy, AC., Fukuda, D., Song, Z., Hothersall, J., Cox, RJ., Willis, CL., Thomas, C. & Simpson, TJ., 1 Jan 2011, In: Angewandte Chemie (International Edition) . 50, 14, p. 3271-3274 4 p.Research output: Contribution to journal › Article
32 Citations (Scopus) -
Manipulation of quorum sensing regulation in Pseudomonas fluorescens NCIMB 10586 to increase mupirocin production.
Hothersall, J., Murphy, AC., Iqbal, Z., Campbell, G., Stephens, E., Wu, J., Cooper, H., Atkinson, S., Williams, P., Crosby, J., Willis, CL., Cox, RJ., Simpson, TJ. & Thomas, C., 12 Feb 2011, In: Applied Microbiology and Biotechnology.Research output: Contribution to journal › Article
7 Citations (Scopus) -
2010
Phosphopantetheinylation and Specificity of Acyl Carrier Proteins in the Mupirocin Biosynthetic Cluster
Shields, JA., Rahman, AS., Arthur, CJ., Crosby, J., Hothersall, J., Simpson, TJ. & Thomas, C., 25 Jan 2010, In: ChemBioChem. 11, 2, p. 248-255 8 p.Research output: Contribution to journal › Article
11 Citations (Scopus) -
Resistance to and synthesis of the antibiotic mupirocin
Thomas, C., Hothersall, J., Willis, CL. & Simpson, TJ., 1 Apr 2010, In: Nature Reviews Microbiology. 8, 4, p. 281-289 9 p.Research output: Contribution to journal › Article
111 Citations (Scopus) -
2009
Genomic and genetic analyses of diversity and plant interactions of Pseudomonas fluorescens
Hothersall, J., Stephens, E. & Thomas, C., 1 Jan 2009, In: Genome Biology. 10, 5, p. R51Research output: Contribution to journal › Article
271 Citations (Scopus) -
2008
In Vivo and In Vitro Trans-Acylation by BryP, the Putative Bryostatin Pathway Acyltransferase Derived from an Uncultured Marine Symbiont
Lopanik, NB., Shields, JA., Buchholz, TJ., Rath, CM., Hothersall, J., Haygood, MG., Hakansson, K., Thomas, C. & Sherman, DH., 24 Nov 2008, In: Chemistry & Biology. 15, 11, p. 1175-1186 12 p.Research output: Contribution to journal › Article
62 Citations (Scopus) -
In vivo mutational analysis of the mupirocin gene cluster reveals labile points in the biosynthetic pathway: the "Leaky Hosepipe" mechanism
Wu, J., Hothersall, J., Mazzetti, C., O'Connell, Y., Shields, JA., Rahman, A., Cox, RJ., Crosby, J., Simpson, TJ., Thomas, C. & Willis, CL., 1 Jan 2008, In: ChemBioChem. 9, 9, p. 1500-1508 9 p.Research output: Contribution to journal › Article
30 Citations (Scopus) -
2007
Mupirocin H, a novel metabolite resulting from mutation of the HMG-CoA synthase analogue, mupH in Pseudomonas fluorescens
Wu, J., Cooper, SM., Cox, RJ., Crosby, J., Crump, MP., Hothersall, J., Simpson, TJ., Thomas, C. & Willis, CL., 1 Jan 2007, In: Chemical Communications. 20, p. 2040-2042 3 p.Research output: Contribution to journal › Article
34 Citations (Scopus) -
Mutational analysis reveals that all tailoring region genes are required for production of polyketide antibiotic mupirocin by Pseudomonas fluorescens: pseudomonic acid B biosynthesis precedes pseudomonic acid A
Hothersall, J., Wu, J., Rahman, A., Shields, J., Haddock, J., Johnson, N., Cooper, SM., Stephens, E., Cox, RJ., Crosby, J., Willis, CL., Simpson, TJ. & Thomas, C., 29 Mar 2007, In: Journal of Biological Chemistry. 282, p. 15451-15461 11 p.Research output: Contribution to journal › Article
28 Citations (Scopus) -
2005
Mupirocin W, a novel pseudomonic acid produced by targeted mutation of the mupirocin biosynthetic gene cluster
Cooper, S., Cox, RJ., Crosby, J., Crump, MP., Hothersall, J., Laosripaiboon, W., Simpson, TJ. & Thomas, C., 1 Jan 2005, In: Chemical Communications. 9, p. 1179-1181 3 p.Research output: Contribution to journal › Article › peer-review
28 Citations (Scopus) -
Shift to pseudomonic acid B production in P-fluorescens NCIMB10586 by mutation of Mupirocin tailoring genes mupO, mupV, and macpE
Cooper, S., Laosripaiboon, W., Rahman, A., Hothersall, J., El-Sayed, AK., Winfield, C., Crosby, J., Cox, RJ., Simpson, TJ. & Thomas, C., 1 Jul 2005, In: Chemistry & Biology. 12, 7, p. 825-833 9 p.Research output: Contribution to journal › Article
22 Citations (Scopus) -
Tandemly duplicated acyl carrier proteins, which increase polyketide antibiotic production, can apparently function either in parallel or in series
Rahman, A., Hothersall, J., Crosby, J., Simpson, TJ. & Thomas, C., 4 Jan 2005, In: Journal of Biological Chemistry. 280, 8, p. 6399-6408 10 p.Research output: Contribution to journal › Article
48 Citations (Scopus) -
2003
Characterization of the mupirocin biosynthesis gene cluster from Pseudomonas fluorescens NCIMB 10586.
El-Sayed, AK., Hothersall, J., Cooper, S., Stephens, E., Simpson, TJ. & Thomas, C., 1 May 2003, In: Chemistry & Biology. 10, 5, p. 419-30 12 p.Research output: Contribution to journal › Article
195 Citations (Scopus) -
2001
Quorum regulation of biosynthesis of polyketide antibiotic, mupirocin, in Pseudomonas fluorescens NC1B10586
El-Sayed, A., Hothersall, J. & Thomas, C., 1 Jan 2001, In: Microbiology. 147, p. 2127-2139 13 p.Research output: Contribution to journal › Article
90 Citations (Scopus)