Helen Cooper

Accepting PhD Students

PhD projects

Dr Cooper’s current research is centred on advanced mass spectrometry techniques for the analysis of biomolecular and chemical structure. Her lab is interested in the development and application of mass spectrometry techniques for the characterisation of biomolecular and non-biomolecular structures. Current work in the laboratory focuses on the following areas:

Development of methods for proteomics
Fundamentals of peptide fragmentation
Analysis of post-translational modifications
Ion mobility spectrometry and mass spectrometry
Direct surface sampling of dried blood spots

Research activity per year

125 Article
9 Abstract
5 Paper
4 Review article
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1 - 50 out of 147 results

2024
Laser capture microdissection and native mass spectrometry for spatially-resolved analysis of intact protein assemblies in tissue †
Hughes, J., Sisley, E. K., Hale, O. J. & Cooper, H. J., 7 Mar 2024, (E-pub ahead of print) In: Chemical Science.
Research output: Contribution to journal › Article › peer-review

Open Access
Mass spectrometry imaging of SOD1 protein-metal complexes in SOD1G93A transgenic mice implicates demetalation with pathology
Hale, O. J., Wells, T. R., Mead, R. J. & Cooper, H. J., 8 Aug 2024, In: Nature Communications. 15, 1, 6518.
Research output: Contribution to journal › Article › peer-review

Open Access
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40 Downloads (Pure)
2023
High-Throughput Deconvolution of Native Protein Mass Spectrometry Imaging Data Sets for Mass Domain Analysis
Hale, O., Cooper, H. J. & Marty, M. T., 19 Sept 2023, In: Analytical Chemistry. 95, 37, p. 14009-14015 7 p.
Research output: Contribution to journal › Article › peer-review

Open Access
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62 Downloads (Pure)
Tissue Washing Improves Native Ambient Mass Spectrometry Detection of Membrane Proteins Directly from Tissue
Sisley, E. K., Hale, O. J., Hughes, J. W. & Cooper, H. J., 26 Jul 2023, In: Journal of the American Chemical Society. 145, 29, p. 15658-15662 5 p.
Research output: Contribution to journal › Article › peer-review

Open Access
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54 Downloads (Pure)
2022
Discovery, validation, and diagnostic ability of multiple protein-based biomarkers in saliva and gingival crevicular fluid to distinguish between health and periodontal diseases
Grant, M., Taylor, J. J., Creese, A., Gowland, C., Burke, B., Doudin, K., Patel, U., Weston, P., Milward, M., Bissett, S. M., Cooper, H., Kooijman, G., Rmaile, A., de Jager, M., Preshaw, P. M. & Chapple, I., 22 Apr 2022, In: Journal of Clinical Periodontology. 49, 7, p. 622-632 11 p.
Research output: Contribution to journal › Article › peer-review

Open Access
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219 Downloads (Pure)
Mass Spectrometry Detection and Imaging of a Non-Covalent Protein-Drug Complex in Tissue from Orally Dosed Rats
Illes-Toth, E., Hale, O. J., Hughes, J. W., Strittmatter, N., Rose, J., Clayton, B., Sargeant, R., Jones, S., Dannhorn, A., Goodwin, R. J. A. & Cooper, H. J., 13 Jul 2022, (E-pub ahead of print) In: Angewandte Chemie (International Edition) . 61, 36, e202202075.
Research output: Contribution to journal › Article › peer-review

Open Access
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128 Downloads (Pure)
Native Ambient Mass Spectrometry Enables Analysis of Intact Endogenous Protein Assemblies up to 145 kDa Directly from Tissue
Hale, O. J., Hughes, J. W., Sisley, E. K. & Cooper, H. J., 12 Apr 2022, In: Analytical Chemistry. 94, 14, p. 5608-5614 7 p.
Research output: Contribution to journal › Article › peer-review

Open Access
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129 Downloads (Pure)
Native Ambient Mass Spectrometry Imaging of Ligand-Bound and Metal-Bound Proteins in Rat Brain
Sisley, E. K., Hale, O. J., Styles, I. B. & Cooper, H. J., 9 Feb 2022, In: Journal of the American Chemical Society. 144, 5, p. 2120-2128 9 p.
Research output: Contribution to journal › Article › peer-review

Open Access
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113 Downloads (Pure)
Native Ambient Mass Spectrometry of an Intact Membrane Protein Assembly and Soluble Protein Assemblies Directly from Lens Tissue
Hale, O. J. & Cooper, H. J., 1 Aug 2022, In: Angewandte Chemie (International Edition) . 61, 31, e202201458.
Research output: Contribution to journal › Article › peer-review

Open Access
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86 Downloads (Pure)
Native ambient mass spectrometry of intact protein assemblies directly from Escherichia coli colonies
Du, Y., May, R. C. & Cooper, H. J., 18 Jun 2022, In: Chemical Communications. 58, 48, p. 6857-6860 4 p.
Research output: Contribution to journal › Article › peer-review

Open Access
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60 Downloads (Pure)
Quantitative Characterization of Three Carbonic Anhydrase Inhibitors by LESA Mass Spectrometry
Illes-Toth, E., Stubbs, C. J., Sisley, E. K., Bellamy-Carter, J., Simmonds, A. L., Mize, T. H., Styles, I. B., Goodwin, R. J. A. & Cooper, H. J., 6 Jul 2022, In: Journal of the American Society for Mass Spectrometry. 33, 7, p. 1168-1175 8 p.
Research output: Contribution to journal › Article › peer-review

Open Access
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98 Downloads (Pure)
2021
Liquid extraction surface analysis mass spectrometry of ESKAPE pathogens
Havlikova, J., May, R., Styles, I. & Cooper, H., 1 Mar 2021, (E-pub ahead of print) In: Journal of the American Society for Mass Spectrometry. 32, 6, p. 1345-1351
Research output: Contribution to journal › Article › peer-review

Open Access
File
196 Downloads (Pure)
Native mass spectrometry imaging of proteins and protein complexes by nano-DESI
Hale, O. & Cooper, H., 16 Mar 2021, In: Analytical Chemistry. 93, 10, p. 4619–4627 9 p.
Research output: Contribution to journal › Article › peer-review

Open Access
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272 Downloads (Pure)
Simultaneous spatial, conformational, and mass analysis of intact proteins and protein assemblies by nano-DESI travelling wave ion mobility mass spectrometry imaging
Hale, O., Hughes, J. & Cooper, H., 12 Jun 2021, (E-pub ahead of print) In: International Journal of Mass Spectrometry. 468, 116656.
Research output: Contribution to journal › Article › peer-review

Open Access
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84 Downloads (Pure)
Structural proteomics and protein complexes – special issue
Cooper, H. J. & Leney, A. C., 14 Nov 2021, In: Proteomics. 21, 21-22, 2 p., 2000286.
Research output: Contribution to journal › Editorial
2020
Comprehensive LESA mass spectrometry imaging of intact proteins by integration of cylindrical FAIMS
Griffiths, R., Hughes, J., Abbatiello, S., Belford, M., Styles, I. & Cooper, H., 18 Feb 2020, In: Analytical Chemistry. 92, 4, p. 2885-2890 6 p.
Research output: Contribution to journal › Article › peer-review

Open Access
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12 Citations (Scopus)

371 Downloads (Pure)
Differential responses to kinase inhibition in FGFR2-addicted triple negative breast cancer cells: a quantitative phosphoproteomics study
Cunningham, D., Almuntafeky, A. R. S., Creese, A., Larkins, K., Zhao, H., Ferguson, H. R., Brookes, K., Dudka, A., Cooper, H. & Heath, J., 14 May 2020, In: Scientific Reports. 10, 1, 7950.
Research output: Contribution to journal › Article › peer-review

Open Access
File
2 Citations (Scopus)

171 Downloads (Pure)
Direct identification of bacterial and human proteins from infected wounds in living 3D skin models
Havlikova, J., May, R. C., Styles, I. B. & Cooper, H. J., 17 Jul 2020, In: Scientific Reports. 10, 1, 11900.
Research output: Contribution to journal › Article › peer-review

Open Access
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2 Citations (Scopus)

256 Downloads (Pure)
Electroporation and mass spectrometry: a new paradigm for in situ analysis of intact proteins direct from living yeast colonies
Kocurek, K., Havlikova, J., Buchan, E., Tanner, A., May, R. & Cooper, H., 4 Feb 2020, In: Analytical Chemistry. 92, 3, p. 2605-2611 7 p.
Research output: Contribution to journal › Article › peer-review

Open Access
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2 Citations (Scopus)

250 Downloads (Pure)
High-field asymmetric waveform ion mobility spectrometry and native mass spectrometry: analysis of intact protein assemblies and protein complexes
Hale, O. J., Illes-Toth, E., Mize, T. H. & Cooper, H. J., 19 May 2020, In: Analytical Chemistry. 92, 10, p. 6811-6816 6 p.
Research output: Contribution to journal › Article › peer-review

Open Access
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9 Citations (Scopus)

222 Downloads (Pure)
In situ mass spectrometry analysis of intact proteins and protein complexes from biological substrates
Hale, O. & Cooper, H., 3 Feb 2020, In: Biochemical Society Transactions. 48, 1, p. 317-326 10 p., BST20190793.
Research output: Contribution to journal › Article › peer-review

Open Access
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3 Citations (Scopus)

186 Downloads (Pure)
LESA Cyclic Ion Mobility Mass Spectrometry of Intact Proteins from Thin Tissue Sections
Sisley, E. K., Ujma, J., Palmer, M., Giles, K., Fernandez-Lima, F. A. & Cooper, H. J., 5 May 2020, In: Analytical Chemistry. 92, 9, p. 6321-6326 6 p.
Research output: Contribution to journal › Article › peer-review

Open Access
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5 Citations (Scopus)

213 Downloads (Pure)
Native LESA TWIMS-MSI: Spatial, Conformational, and Mass Analysis of Proteins and Protein Complexes
Hale, O. J., Sisley, E. K., Griffiths, R. L., Styles, I. B. & Cooper, H. J., 1 Apr 2020, In: Journal of the American Society for Mass Spectrometry. 31, 4, p. 873-879 7 p.
Research output: Contribution to journal › Article › peer-review

Open Access
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7 Citations (Scopus)

289 Downloads (Pure)
Native Mass Spectrometry Imaging and In Situ Top-Down Identification of Intact Proteins Directly from Tissue
Hale, O. J. & Cooper, H. J., 21 Aug 2020, (E-pub ahead of print) In: Journal of the American Society for Mass Spectrometry. 31, 12, p. 2531-2537
Research output: Contribution to journal › Article › peer-review

Open Access
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5 Citations (Scopus)

249 Downloads (Pure)
Self-Incompatibility Triggers Irreversible Oxidative Modification of Proteins in Incompatible Pollen
Haque, T., Eaves, D. J., Lin, Z., Zampronio, C. G., Cooper, H. J., Bosch, M., Smirnoff, N. & Franklin-tong, V. E., 1 Jul 2020, In: Plant Physiology. 183, 3, p. 1391-1404
Research output: Contribution to journal › Article › peer-review

Open Access
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218 Downloads (Pure)
Structural analysis of 14-3-3-ζ-derived phosphopeptides using electron capture dissociation mass spectrometry, traveling wave ion mobility spectrometry, and molecular modeling
Simmonds, A., Lopez-Clavijo, A., Winn, P., Russell, D., Styles, I. & Cooper, H., 23 Jan 2020, In: Journal of Physical Chemistry B. 124, 3, p. 461-469 9 p.
Research output: Contribution to journal › Article › peer-review

Open Access
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1 Citation (Scopus)

262 Downloads (Pure)
2019
Application of high-field asymmetric waveform ion mobility separation to LESA mass spectrometry of bacteria
Kocurek, K., May, R. & Cooper, H., 2 Apr 2019, In: Analytical Chemistry. 91, 7, p. 4755-4761 7 p.
Research output: Contribution to journal › Article › peer-review

Open Access
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7 Citations (Scopus)

274 Downloads (Pure)
Direct Mass Spectrometry Analysis of Protein Complexes and Intact Proteins up to >70 kDa from Tissue
Griffiths, R., Konijnenberg, A., Viner, R. & Cooper, H., 4 Jun 2019, In: Analytical Chemistry. 91, 11, p. 6962-6966 5 p.
Research output: Contribution to journal › Article › peer-review

Open Access
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10 Citations (Scopus)

325 Downloads (Pure)
In situ analysis of intact proteins by ion mobility mass spectrometry
Sisley, E., Illes-Toth, T. E. & Cooper, H., 31 May 2019, (E-pub ahead of print) In: Trends in Analytical Chemistry.
Research output: Contribution to journal › Article › peer-review

Open Access
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5 Citations (Scopus)

211 Downloads (Pure)
Investigation of the 12-month stability and detectable metabolomes of dried blood and urine spots applying untargeted UHPLC-MS metabolomic assays
Palmer, E., Cooper, H. & Dunn, W., 19 Nov 2019, In: Analytical Chemistry. 91, 22, p. 14306−14313 8 p.
Research output: Contribution to journal › Article › peer-review
10 Citations (Scopus)
Native mass spectrometry imaging of intact proteins and protein complexes in thin tissue sections
Griffiths, R., Sisley, E., Lopez Clavijo, A., Simmonds, A., Styles, I. & Cooper, H., Mar 2019, In: International Journal of Mass Spectrometry. 437, p. 23-29 7 p.
Research output: Contribution to journal › Article › peer-review

Open Access
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24 Citations (Scopus)

259 Downloads (Pure)
Probing the Fundamentals of Native Liquid Extraction Surface Analysis Mass Spectrometry of Proteins: Can Proteins Refold during Extraction?
Illes-Toth, E. & Cooper, H. J., 1 Oct 2019, In: Analytical Chemistry. 91, 19, p. 12246-12254 9 p.
Research output: Contribution to journal › Article › peer-review

Open Access
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3 Citations (Scopus)

206 Downloads (Pure)
Quantitative Imaging of Proteins in Tissue by Stable Isotope Labeled Mimetic Liquid Extraction Surface Analysis Mass Spectrometry
Havlikova, J., Randall, E. C., Griffiths, R. L., Swales, J. G., Goodwin, R. J. A., Bunch, J., Styles, I. B. & Cooper, H. J., 19 Nov 2019, In: Analytical Chemistry. 91, 22, p. 14198-14202 5 p.
Research output: Contribution to journal › Article › peer-review

Open Access
File
8 Citations (Scopus)

251 Downloads (Pure)
2018
Ambient ionization mass spectrometry for in situ analysis of intact proteins
Kocurek, K. I., Griffiths, R. L. & Cooper, H. J., 24 May 2018, (E-pub ahead of print) In: Journal of mass spectrometry : JMS.
Research output: Contribution to journal › Article › peer-review

Open Access
File
9 Citations (Scopus)

218 Downloads (Pure)
Ambient surface mass spectrometry-ion mobility spectrometry of intact proteins
Griffiths, R., Kocurek, K. & Cooper, H., Feb 2018, In: Current Opinion in Chemical Biology. 42, p. 67-75
Research output: Contribution to journal › Article › peer-review

Open Access
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5 Citations (Scopus)

167 Downloads (Pure)
High Field Asymmetric Waveform Ion Mobility Spectrometry in Non-targeted Bottom-up Proteomics of Dried Blood Spots
Rosting, C., Yu, J. & Cooper, H. J., 30 Apr 2018, In: Journal of Proteome Research. 17, 6, p. 1997-2004 8 p.
Research output: Contribution to journal › Article › peer-review

Open Access
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13 Citations (Scopus)

430 Downloads (Pure)
LESA MS imaging of heat preserved and frozen tissue: Benefits of multi-step static FAIMS
Griffiths, R., Simmonds, A., Swales, J., Goodwin, R. & Cooper, H., 15 Oct 2018, In: Analytical Chemistry.
Research output: Contribution to journal › Article › peer-review

Open Access
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14 Citations (Scopus)

359 Downloads (Pure)
Liquid Extraction Surface Analysis (LESA) Electron-Induced Dissociation and Collision-Induced Dissociation Mass Spectrometry of Small Molecule Drug Compounds
Lopez-Clavijo, A. F., Griffiths, R. L., Goodwin, R. J. A. & Cooper, H. J., Nov 2018, In: Journal of the American Society for Mass Spectrometry. 29, 11, p. 2218-2226 9 p.
Research output: Contribution to journal › Article › peer-review

Open Access
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6 Citations (Scopus)

254 Downloads (Pure)
2017
Identification of Phosphorylation Sites Altering Pollen Soluble Inorganic Pyrophosphatase Activity
Eaves, D. J., Haque, T., Tudor, R. L., Barron, Y., Zampronio, C. G., Cotton, N. P. J., de Graaf, B. H. J., White, S. A., Cooper, H., Franklin, F. C. H., Harper, J. F. & Franklin-Tong, V. E., Mar 2017, In: Plant Physiology. 173, 3, p. 1606-1616
Research output: Contribution to journal › Article › peer-review

Open Access
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6 Citations (Scopus)

257 Downloads (Pure)
Raster mode continuous-flow liquid microjunction mass spectrometry imaging of proteins in thin tissue sections
Griffiths, R., Randall, E., Race, A., Bunch, J. & Cooper, H., 6 Jun 2017, In: Analytical Chemistry. 89, 11, p. 5683–5687
Research output: Contribution to journal › Article › peer-review

Open Access
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21 Citations (Scopus)

265 Downloads (Pure)
Sub-critical water hydrolysis of peptides: Amino acid side-chain modifications
Powell, T., Bowra, S. & Cooper, H., Sept 2017, In: Journal of the American Society for Mass Spectrometry. 28, 9, p. 1775–1786
Research output: Contribution to journal › Article › peer-review

Open Access
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6 Citations (Scopus)

170 Downloads (Pure)
Top-down LESA mass spectrometry protein analysis of gram-positive and gram-negative bacteria
May, R., Cooper, H., Kocurek, K., Bunch, J. & Stones, L., Oct 2017, In: Journal of the American Society for Mass Spectrometry. 28, 10, p. 2066–2077
Research output: Contribution to journal › Article › peer-review

Open Access
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20 Citations (Scopus)

230 Downloads (Pure)
2016
Direct Tissue Profiling of Protein Complexes: Toward Native Mass Spectrometry Imaging
Griffiths, R. & Cooper, H., Jan 2016, In: Analytical Chemistry. 88, 1, p. 606-9
Research output: Contribution to journal › Article › peer-review

Open Access
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23 Citations (Scopus)

230 Downloads (Pure)
LESA FAIMS mass spectrometry for the spatial profiling of proteins from tissue
Griffiths, R., Creese, A., Race, A., Bunch, J. & Cooper, H., 5 Jul 2016, In: Analytical Chemistry. 88, 13, p. 6758–6766
Research output: Contribution to journal › Article › peer-review

Open Access
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47 Citations (Scopus)

268 Downloads (Pure)
Liquid Extraction Surface Analysis for Native Mass Spectrometry: Protein Complexes and Ligand Binding
Mikhailov, V., Griffiths, R. & Cooper, H., 21 Sept 2016, (E-pub ahead of print) In: International Journal of Mass Spectrometry.
Research output: Contribution to journal › Article › peer-review

Open Access
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22 Citations (Scopus)

451 Downloads (Pure)
MALDI imaging of liquid extraction surface analysis sampled tissue
Randall, E., Race, A., Cooper, H. & Bunch, J., 6 Sept 2016, In: Analytical Chemistry. 88, 17, p. 8433–8440
Research output: Contribution to journal › Article › peer-review

Open Access
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17 Citations (Scopus)

209 Downloads (Pure)
Separation of cis and trans isomers of polyproline by FAIMS mass spectrometry
Creese, A. & Cooper, H., Dec 2016, In: Journal of the American Society for Mass Spectrometry. 27, 12, p. 2071–2074
Research output: Contribution to journal › Article › peer-review

Open Access
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3 Citations (Scopus)

188 Downloads (Pure)
Sub-critical water processing of proteins: An alternative to enzymatic digestion?
Powell, T., Bowra, S. & Cooper, H., 2016, In: Analytical Chemistry. 88, 12, p. 6425–6432
Research output: Contribution to journal › Article › peer-review

Open Access
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33 Citations (Scopus)

235 Downloads (Pure)
To what extent is FAIMS beneficial in the analysis of proteins?
Cooper, H. J., Apr 2016, In: Journal of the American Society for Mass Spectrometry. 27, 4, p. 566-577
Research output: Contribution to journal › Article › peer-review

Open Access
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28 Citations (Scopus)

170 Downloads (Pure)
2015
Comprehensive mapping of O-glycosylation in flagellin from Campylobacter jejuni 11168: A multienzyme differential ion mobility mass spectrometry approach
Ulasi, G. N., Creese, A. J., Hui, S. X., Penn, C. W. & Cooper, H. J., 6 Aug 2015, In: Proteomics. 15, 16, p. 2733-2745 13 p.
Research output: Contribution to journal › Article › peer-review

Open Access
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22 Citations (Scopus)

175 Downloads (Pure)

Helen Cooper

Research output

Search results